Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the International Symposium on Plant Biotechnology |
| Subtitle of host publication | From Bench to Commercialization, IHC 2006 |
| Publisher | International Society for Horticultural Science |
| Pages | 219-224 |
| Number of pages | 6 |
| ISBN (print) | 9789066056909 |
| Publication status | Published - Dec 2007 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 764 |
| ISSN (Print) | 0567-7572 |
Abstract
Embryogenic culture was initiated from phylloclade explants of Schlumbergera truncata 'Russian Dancer'. Callus developed on phylloclade explants when grown on MS medium containing cytokinins including TDZ, BA and zeatin. Callus cultures were periodically sub-cultured on the same medium over a period of 16 months. Callus grown in SH based liquid media supplemented with 7.0 μM kinetin and transferred onto solid MS based medium devoid of hormones developed new friable embryogenic callus. Somatic embryos differentiated from this type of callus after three and half months from the start of the embryogenesis experiment. 70% germination was recorded for dicotyledonous embryos and plants derived from somatic embryos showed 81% survival when transferred to greenhouse conditions. Histological studies showed the discrete vascular system of the somatic embryos. It was also possible to characterize the distinct root and shoot poles.
Keywords
- Christmas cactus, Multiple cotyledons, Secondary embryos
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the International Symposium on Plant Biotechnology: From Bench to Commercialization, IHC 2006. International Society for Horticultural Science, 2007. p. 219-224 (Acta Horticulturae; Vol. 764).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Plant regeneration via somatic embryogenesis in Schlumbergera truncata
AU - Al-Ramamneh, E.
AU - Sriskandarajah, S.
AU - Serek, M.
PY - 2007/12
Y1 - 2007/12
N2 - Embryogenic culture was initiated from phylloclade explants of Schlumbergera truncata 'Russian Dancer'. Callus developed on phylloclade explants when grown on MS medium containing cytokinins including TDZ, BA and zeatin. Callus cultures were periodically sub-cultured on the same medium over a period of 16 months. Callus grown in SH based liquid media supplemented with 7.0 μM kinetin and transferred onto solid MS based medium devoid of hormones developed new friable embryogenic callus. Somatic embryos differentiated from this type of callus after three and half months from the start of the embryogenesis experiment. 70% germination was recorded for dicotyledonous embryos and plants derived from somatic embryos showed 81% survival when transferred to greenhouse conditions. Histological studies showed the discrete vascular system of the somatic embryos. It was also possible to characterize the distinct root and shoot poles.
AB - Embryogenic culture was initiated from phylloclade explants of Schlumbergera truncata 'Russian Dancer'. Callus developed on phylloclade explants when grown on MS medium containing cytokinins including TDZ, BA and zeatin. Callus cultures were periodically sub-cultured on the same medium over a period of 16 months. Callus grown in SH based liquid media supplemented with 7.0 μM kinetin and transferred onto solid MS based medium devoid of hormones developed new friable embryogenic callus. Somatic embryos differentiated from this type of callus after three and half months from the start of the embryogenesis experiment. 70% germination was recorded for dicotyledonous embryos and plants derived from somatic embryos showed 81% survival when transferred to greenhouse conditions. Histological studies showed the discrete vascular system of the somatic embryos. It was also possible to characterize the distinct root and shoot poles.
KW - Christmas cactus
KW - Multiple cotyledons
KW - Secondary embryos
UR - http://www.scopus.com/inward/record.url?scp=42149142982&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2007.764.29
DO - 10.17660/ActaHortic.2007.764.29
M3 - Conference contribution
AN - SCOPUS:42149142982
SN - 9789066056909
T3 - Acta Horticulturae
SP - 219
EP - 224
BT - Proceedings of the International Symposium on Plant Biotechnology
PB - International Society for Horticultural Science
ER -