Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding |
| Publisher | International Society for Horticultural Science |
| Pages | 397-400 |
| Number of pages | 4 |
| ISBN (print) | 906605719X, 9789066057197 |
| Publication status | Published - Nov 2006 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 725 I |
| ISSN (Print) | 0567-7572 |
Abstract
In order to develop an in vitro regeneration protocol for different Streptocarpus species, we investigated the effects of three factors: surface sterilization, regeneration medium and explant type. For surface sterilization the influence of an ethanol (70%) treatment for 30 s was tested, before the explants were immersed in 1.5% sodium hypochlorite for 10 min. Our experiments showed no significant difference regarding contamination of explants between the surface sterilization with and without ethanol. On the other hand, the treatment with 70% ethanol led to damage of the leaf explants regarding regeneration, especially the number of shootlets. The variation of indole acetic acid (IAA) and benzylaminopurine (BAP) concentrations in the regeneration medium revealed, that a combination of 1.0 mg L-1 IAA and 1.0 mg L-1 BAP was most efficient for shoot regeneration in three species (S1, S3, S4). However, the best quality and regeneration response of the fourth species (S7) was obtained with 1.0 mg L-1 IAA and 0.1 mg L -1 BAP. The differences of the most suitable combination of phytohormones showed, that it was not possible to designate a uniform optimal medium for all of Streptocarpus species. In addition, different explant types were compared. Taking into consideration the regeneration intensity, the expenditure of time for explant preparation and material costs, we concluded that the most suitable explant type were leaf blade explants.
Keywords
- BAP, Cape primrose, Gesneriaceae, IAA, Surface sterilization
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding. International Society for Horticultural Science, 2006. p. 397-400 (Acta Horticulturae; Vol. 725 I).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Plant regeneration from different explant types of Streptocarpus Spec. in vitro
AU - Afkhami-Sarvestani, R.
AU - Serek, M.
AU - Winkelmann, T.
PY - 2006/11
Y1 - 2006/11
N2 - In order to develop an in vitro regeneration protocol for different Streptocarpus species, we investigated the effects of three factors: surface sterilization, regeneration medium and explant type. For surface sterilization the influence of an ethanol (70%) treatment for 30 s was tested, before the explants were immersed in 1.5% sodium hypochlorite for 10 min. Our experiments showed no significant difference regarding contamination of explants between the surface sterilization with and without ethanol. On the other hand, the treatment with 70% ethanol led to damage of the leaf explants regarding regeneration, especially the number of shootlets. The variation of indole acetic acid (IAA) and benzylaminopurine (BAP) concentrations in the regeneration medium revealed, that a combination of 1.0 mg L-1 IAA and 1.0 mg L-1 BAP was most efficient for shoot regeneration in three species (S1, S3, S4). However, the best quality and regeneration response of the fourth species (S7) was obtained with 1.0 mg L-1 IAA and 0.1 mg L -1 BAP. The differences of the most suitable combination of phytohormones showed, that it was not possible to designate a uniform optimal medium for all of Streptocarpus species. In addition, different explant types were compared. Taking into consideration the regeneration intensity, the expenditure of time for explant preparation and material costs, we concluded that the most suitable explant type were leaf blade explants.
AB - In order to develop an in vitro regeneration protocol for different Streptocarpus species, we investigated the effects of three factors: surface sterilization, regeneration medium and explant type. For surface sterilization the influence of an ethanol (70%) treatment for 30 s was tested, before the explants were immersed in 1.5% sodium hypochlorite for 10 min. Our experiments showed no significant difference regarding contamination of explants between the surface sterilization with and without ethanol. On the other hand, the treatment with 70% ethanol led to damage of the leaf explants regarding regeneration, especially the number of shootlets. The variation of indole acetic acid (IAA) and benzylaminopurine (BAP) concentrations in the regeneration medium revealed, that a combination of 1.0 mg L-1 IAA and 1.0 mg L-1 BAP was most efficient for shoot regeneration in three species (S1, S3, S4). However, the best quality and regeneration response of the fourth species (S7) was obtained with 1.0 mg L-1 IAA and 0.1 mg L -1 BAP. The differences of the most suitable combination of phytohormones showed, that it was not possible to designate a uniform optimal medium for all of Streptocarpus species. In addition, different explant types were compared. Taking into consideration the regeneration intensity, the expenditure of time for explant preparation and material costs, we concluded that the most suitable explant type were leaf blade explants.
KW - BAP
KW - Cape primrose
KW - Gesneriaceae
KW - IAA
KW - Surface sterilization
UR - http://www.scopus.com/inward/record.url?scp=33846469990&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2006.725.52
DO - 10.17660/ActaHortic.2006.725.52
M3 - Conference contribution
AN - SCOPUS:33846469990
SN - 906605719X
SN - 9789066057197
T3 - Acta Horticulturae
SP - 397
EP - 400
BT - Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding
PB - International Society for Horticultural Science
ER -