Details
| Original language | English |
|---|---|
| Pages (from-to) | 2964-77 |
| Number of pages | 14 |
| Journal | The plant cell |
| Volume | 23 |
| Issue number | 8 |
| Publication status | Published - Aug 2011 |
Abstract
Within dense plant populations, strong light quality gradients cause unbalanced excitation of the two photosystems resulting in reduced photosynthetic efficiency. Plants redirect such imbalances by structural rearrangements of the photosynthetic apparatus via state transitions and photosystem stoichiometry adjustments. However, less is known about the function of photosystem II (PSII) supercomplexes in this context. Here, we show in Arabidopsis thaliana that PSII supercomplex remodeling precedes and facilitates state transitions. Intriguingly, the remodeling occurs in the short term, paralleling state transitions, but is also present in a state transition-deficient mutant, indicating that PSII supercomplex generation is independently regulated and does not require light-harvesting complex phosphorylation and movement. Instead, PSII supercomplex remodeling involves reversible phosphorylation of PSII core subunits (preferentially of CP43) and requires the luminal PSII subunit Psb27 for general formation and structural stabilization. Arabidopsis knockout mutants lacking Psb27 display highly accelerated state transitions, indicating that release of PSII supercomplexes is required for phosphorylation and subsequent movement of the antenna. Downregulation of PSII supercomplex number by physiological light treatments also results in acceleration of state transitions confirming the genetic analyses. Thus, supercomplex remodeling is a prerequisite and an important kinetic determinant of state transitions.
Keywords
- Arabidopsis/genetics, Arabidopsis Proteins/genetics, Chlorophyll/metabolism, Chloroplasts/radiation effects, Down-Regulation, Electron Transport, Fluorescence, Light, Light-Harvesting Protein Complexes/genetics, Microscopy, Electron, Transmission, Phosphorylation, Photosynthesis/physiology, Photosystem II Protein Complex/genetics, Plant Leaves/genetics, Sequence Deletion, Thylakoids/metabolism
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In: The plant cell, Vol. 23, No. 8, 08.2011, p. 2964-77.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Photosystem II supercomplex remodeling serves as an entry mechanism for state transitions in Arabidopsis
AU - Dietzel, Lars
AU - Bräutigam, Katharina
AU - Steiner, Sebastian
AU - Schüffler, Kristin
AU - Lepetit, Bernard
AU - Grimm, Bernhard
AU - Schöttler, Mark Aurel
AU - Pfannschmidt, Thomas
PY - 2011/8
Y1 - 2011/8
N2 - Within dense plant populations, strong light quality gradients cause unbalanced excitation of the two photosystems resulting in reduced photosynthetic efficiency. Plants redirect such imbalances by structural rearrangements of the photosynthetic apparatus via state transitions and photosystem stoichiometry adjustments. However, less is known about the function of photosystem II (PSII) supercomplexes in this context. Here, we show in Arabidopsis thaliana that PSII supercomplex remodeling precedes and facilitates state transitions. Intriguingly, the remodeling occurs in the short term, paralleling state transitions, but is also present in a state transition-deficient mutant, indicating that PSII supercomplex generation is independently regulated and does not require light-harvesting complex phosphorylation and movement. Instead, PSII supercomplex remodeling involves reversible phosphorylation of PSII core subunits (preferentially of CP43) and requires the luminal PSII subunit Psb27 for general formation and structural stabilization. Arabidopsis knockout mutants lacking Psb27 display highly accelerated state transitions, indicating that release of PSII supercomplexes is required for phosphorylation and subsequent movement of the antenna. Downregulation of PSII supercomplex number by physiological light treatments also results in acceleration of state transitions confirming the genetic analyses. Thus, supercomplex remodeling is a prerequisite and an important kinetic determinant of state transitions.
AB - Within dense plant populations, strong light quality gradients cause unbalanced excitation of the two photosystems resulting in reduced photosynthetic efficiency. Plants redirect such imbalances by structural rearrangements of the photosynthetic apparatus via state transitions and photosystem stoichiometry adjustments. However, less is known about the function of photosystem II (PSII) supercomplexes in this context. Here, we show in Arabidopsis thaliana that PSII supercomplex remodeling precedes and facilitates state transitions. Intriguingly, the remodeling occurs in the short term, paralleling state transitions, but is also present in a state transition-deficient mutant, indicating that PSII supercomplex generation is independently regulated and does not require light-harvesting complex phosphorylation and movement. Instead, PSII supercomplex remodeling involves reversible phosphorylation of PSII core subunits (preferentially of CP43) and requires the luminal PSII subunit Psb27 for general formation and structural stabilization. Arabidopsis knockout mutants lacking Psb27 display highly accelerated state transitions, indicating that release of PSII supercomplexes is required for phosphorylation and subsequent movement of the antenna. Downregulation of PSII supercomplex number by physiological light treatments also results in acceleration of state transitions confirming the genetic analyses. Thus, supercomplex remodeling is a prerequisite and an important kinetic determinant of state transitions.
KW - Arabidopsis/genetics
KW - Arabidopsis Proteins/genetics
KW - Chlorophyll/metabolism
KW - Chloroplasts/radiation effects
KW - Down-Regulation
KW - Electron Transport
KW - Fluorescence
KW - Light
KW - Light-Harvesting Protein Complexes/genetics
KW - Microscopy, Electron, Transmission
KW - Phosphorylation
KW - Photosynthesis/physiology
KW - Photosystem II Protein Complex/genetics
KW - Plant Leaves/genetics
KW - Sequence Deletion
KW - Thylakoids/metabolism
U2 - 10.1105/tpc.111.087049
DO - 10.1105/tpc.111.087049
M3 - Article
C2 - 21880991
VL - 23
SP - 2964
EP - 2977
JO - The plant cell
JF - The plant cell
SN - 1040-4651
IS - 8
ER -