Details
| Original language | English |
|---|---|
| Pages (from-to) | 46594-600 |
| Number of pages | 7 |
| Journal | Journal of Biological Chemistry |
| Volume | 277 |
| Issue number | 48 |
| Publication status | Published - 29 Nov 2002 |
Abstract
The influence of photosynthetic electron flow in chloroplasts on the expression and enzyme activity of the cytosolic nitrate reductase (NR) was studied. Using light sources that predominantly excite either photosystem I (PSI) or photosystem II (PSII), we modulated photosynthetic electron transport in tobacco, Arabidopsis, and Lemna sprouts. In all instances, oxidation of components of photosynthetic electron flow by PSI light correlated with an increase in NR activity and/or transcription. This is confirmed by experiments with electron transport inhibitors 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. In addition, a Lemna mutant deficient in the cytochrome b(6)/f complex failed to respond to the different light sources and exhibited a constitutively high level of NR activity. These data indicate that NR is activated by the oxidized state of an electron transport component located after the plastoquinone pool. An involvement of the cytoplasmic photoreceptor phytochrome A in this light regulation could be excluded, since an Arabidopsis phytochrome A mutant exhibited a wild-type like response. The observation that NR activity in the cytoplasm and the expression of its gene in the nucleus is controlled by signals from photosynthetic electron flow adds a new facet to the intracellular cross-talk between chloroplasts and the nucleus.
Keywords
- Arabidopsis/enzymology, Artificial Gene Fusion, Base Sequence, DNA Primers, Electron Transport, Gene Expression Regulation, Enzymologic, Nitrate Reductase, Nitrate Reductases/genetics, Oxidation-Reduction, Photosynthesis, Plants, Genetically Modified, Promoter Regions, Genetic, RNA, Messenger/genetics, Tobacco/enzymology
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In: Journal of Biological Chemistry, Vol. 277, No. 48, 29.11.2002, p. 46594-600.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Photosynthetic electron transport determines nitrate reductase gene expression and activity in higher plants
AU - Sherameti, Irena
AU - Sopory, Sudhir K
AU - Trebicka, Artan
AU - Pfannschmidt, Thomas
AU - Oelmuller, Ralf
PY - 2002/11/29
Y1 - 2002/11/29
N2 - The influence of photosynthetic electron flow in chloroplasts on the expression and enzyme activity of the cytosolic nitrate reductase (NR) was studied. Using light sources that predominantly excite either photosystem I (PSI) or photosystem II (PSII), we modulated photosynthetic electron transport in tobacco, Arabidopsis, and Lemna sprouts. In all instances, oxidation of components of photosynthetic electron flow by PSI light correlated with an increase in NR activity and/or transcription. This is confirmed by experiments with electron transport inhibitors 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. In addition, a Lemna mutant deficient in the cytochrome b(6)/f complex failed to respond to the different light sources and exhibited a constitutively high level of NR activity. These data indicate that NR is activated by the oxidized state of an electron transport component located after the plastoquinone pool. An involvement of the cytoplasmic photoreceptor phytochrome A in this light regulation could be excluded, since an Arabidopsis phytochrome A mutant exhibited a wild-type like response. The observation that NR activity in the cytoplasm and the expression of its gene in the nucleus is controlled by signals from photosynthetic electron flow adds a new facet to the intracellular cross-talk between chloroplasts and the nucleus.
AB - The influence of photosynthetic electron flow in chloroplasts on the expression and enzyme activity of the cytosolic nitrate reductase (NR) was studied. Using light sources that predominantly excite either photosystem I (PSI) or photosystem II (PSII), we modulated photosynthetic electron transport in tobacco, Arabidopsis, and Lemna sprouts. In all instances, oxidation of components of photosynthetic electron flow by PSI light correlated with an increase in NR activity and/or transcription. This is confirmed by experiments with electron transport inhibitors 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. In addition, a Lemna mutant deficient in the cytochrome b(6)/f complex failed to respond to the different light sources and exhibited a constitutively high level of NR activity. These data indicate that NR is activated by the oxidized state of an electron transport component located after the plastoquinone pool. An involvement of the cytoplasmic photoreceptor phytochrome A in this light regulation could be excluded, since an Arabidopsis phytochrome A mutant exhibited a wild-type like response. The observation that NR activity in the cytoplasm and the expression of its gene in the nucleus is controlled by signals from photosynthetic electron flow adds a new facet to the intracellular cross-talk between chloroplasts and the nucleus.
KW - Arabidopsis/enzymology
KW - Artificial Gene Fusion
KW - Base Sequence
KW - DNA Primers
KW - Electron Transport
KW - Gene Expression Regulation, Enzymologic
KW - Nitrate Reductase
KW - Nitrate Reductases/genetics
KW - Oxidation-Reduction
KW - Photosynthesis
KW - Plants, Genetically Modified
KW - Promoter Regions, Genetic
KW - RNA, Messenger/genetics
KW - Tobacco/enzymology
U2 - 10.1074/jbc.M202924200
DO - 10.1074/jbc.M202924200
M3 - Article
C2 - 12244040
VL - 277
SP - 46594
EP - 46600
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 48
ER -