Details
| Original language | English |
|---|---|
| Pages (from-to) | 1045-54 |
| Number of pages | 10 |
| Journal | Plant & cell physiology |
| Volume | 41 |
| Issue number | 9 |
| Publication status | Published - Sept 2000 |
Abstract
Plants respond to changing light conditions by altering the stoichiometry between components of the photosynthetic electron transport chain of chloroplast thylakoids. We measured specific run-on transcription of the chloroplast genes psaB, psbA and rbcL in pea (Pisum sativum L.) seedlings grown under three different conditions of illumination: light selective for photosystem I (PSI-light); light selective for photosystem II (PSII-light); and a combination of PSI- and PSII-light (mixed light, ML). The transcriptional rate of the psaB gene increased under PSII-light and decreased under PSI-light, while the transcriptional rates of the psbA and rbcL genes were affected only in a non-specific way. Similar effects also occurred in plants grown under ML and switched to either PSI- or PSII-light for 4 h. Addition of the inhibitors of photosynthetic electron transport 3-(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) influenced psaB transcription in isolated, illuminated chloroplasts: DCMU addition resulted in oxidation of the plastoquinone pool and decreased transcription of psaB; DBMIB addition resulted in reduction of the plastoquinone pool and increased transcription of psaB. The experimental results obtained in vivo and in vitro provide evidence for coupling between the redox state of plastoquinone and the rate of transcription of the psaB gene in pea.
Keywords
- Chloroplasts/metabolism, Dibromothymoquinone/pharmacology, Diuron/pharmacology, Electron Transport, Gene Expression Regulation, Plant/drug effects, Kinetics, Light, Membrane Proteins/genetics, Oxidation-Reduction, Peas/genetics, Photosynthesis, Photosynthetic Reaction Center Complex Proteins/genetics, Photosystem I Protein Complex, Photosystem II Protein Complex, Plant Proteins/genetics, Plastoquinone/metabolism, Ribulose-Bisphosphate Carboxylase, Transcription, Genetic/drug effects
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In: Plant & cell physiology, Vol. 41, No. 9, 09.2000, p. 1045-54.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Photosynthetic electron flow regulates transcription of the psaB gene in pea (Pisum sativum L.) chloroplasts through the redox state of the plastoquinone pool
AU - Tullberg, A
AU - Alexciev, K
AU - Pfannschmidt, T
AU - Allen, J F
PY - 2000/9
Y1 - 2000/9
N2 - Plants respond to changing light conditions by altering the stoichiometry between components of the photosynthetic electron transport chain of chloroplast thylakoids. We measured specific run-on transcription of the chloroplast genes psaB, psbA and rbcL in pea (Pisum sativum L.) seedlings grown under three different conditions of illumination: light selective for photosystem I (PSI-light); light selective for photosystem II (PSII-light); and a combination of PSI- and PSII-light (mixed light, ML). The transcriptional rate of the psaB gene increased under PSII-light and decreased under PSI-light, while the transcriptional rates of the psbA and rbcL genes were affected only in a non-specific way. Similar effects also occurred in plants grown under ML and switched to either PSI- or PSII-light for 4 h. Addition of the inhibitors of photosynthetic electron transport 3-(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) influenced psaB transcription in isolated, illuminated chloroplasts: DCMU addition resulted in oxidation of the plastoquinone pool and decreased transcription of psaB; DBMIB addition resulted in reduction of the plastoquinone pool and increased transcription of psaB. The experimental results obtained in vivo and in vitro provide evidence for coupling between the redox state of plastoquinone and the rate of transcription of the psaB gene in pea.
AB - Plants respond to changing light conditions by altering the stoichiometry between components of the photosynthetic electron transport chain of chloroplast thylakoids. We measured specific run-on transcription of the chloroplast genes psaB, psbA and rbcL in pea (Pisum sativum L.) seedlings grown under three different conditions of illumination: light selective for photosystem I (PSI-light); light selective for photosystem II (PSII-light); and a combination of PSI- and PSII-light (mixed light, ML). The transcriptional rate of the psaB gene increased under PSII-light and decreased under PSI-light, while the transcriptional rates of the psbA and rbcL genes were affected only in a non-specific way. Similar effects also occurred in plants grown under ML and switched to either PSI- or PSII-light for 4 h. Addition of the inhibitors of photosynthetic electron transport 3-(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) influenced psaB transcription in isolated, illuminated chloroplasts: DCMU addition resulted in oxidation of the plastoquinone pool and decreased transcription of psaB; DBMIB addition resulted in reduction of the plastoquinone pool and increased transcription of psaB. The experimental results obtained in vivo and in vitro provide evidence for coupling between the redox state of plastoquinone and the rate of transcription of the psaB gene in pea.
KW - Chloroplasts/metabolism
KW - Dibromothymoquinone/pharmacology
KW - Diuron/pharmacology
KW - Electron Transport
KW - Gene Expression Regulation, Plant/drug effects
KW - Kinetics
KW - Light
KW - Membrane Proteins/genetics
KW - Oxidation-Reduction
KW - Peas/genetics
KW - Photosynthesis
KW - Photosynthetic Reaction Center Complex Proteins/genetics
KW - Photosystem I Protein Complex
KW - Photosystem II Protein Complex
KW - Plant Proteins/genetics
KW - Plastoquinone/metabolism
KW - Ribulose-Bisphosphate Carboxylase
KW - Transcription, Genetic/drug effects
U2 - 10.1093/pcp/pcd031
DO - 10.1093/pcp/pcd031
M3 - Article
C2 - 11100777
VL - 41
SP - 1045
EP - 1054
JO - Plant & cell physiology
JF - Plant & cell physiology
SN - 0032-0781
IS - 9
ER -