Details
| Original language | English |
|---|---|
| Article number | 1077 |
| Journal | Polymers |
| Volume | 12 |
| Issue number | 5 |
| Publication status | Published - 8 May 2020 |
Abstract
Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.
Keywords
- Heparin, Interpolyelectrolyte complex, PDNA, Photosensitive, Poly-l-lysine, SiRNA
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Polymers and Plastics
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In: Polymers, Vol. 12, No. 5, 1077, 08.05.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Photosensitive poly-l-lysine/heparin interpolyelectrolyte complexes for delivery of genetic drugs
AU - Korzhikov-Vlakh, Viktor
AU - Katernuk, Iuliia
AU - Pilipenko, Iuliia
AU - Lavrentieva, Antonina
AU - Guryanov, Ivan
AU - Sharoyko, Vladimir
AU - Manshina, Alina A.
AU - Tennikova, Tatiana B.
N1 - Funding information: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German-Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany). The authors are grateful to: Arto Urtti and Marika Rupponen, University of Eastern Finland, as well as Thomas Scheper, Institute of Technical Chemistry, Leibniz University for valuable discussions and help. The authors also would like to thank the Magnetic Resonance Research Centre and Centre for Molecular and Cell Technologies of St. Petersburg State University for NMR and TEM investigations, respectively Funding: The financial support was provided by Russian Science Foundation (project no. 19-73-10045). Ms. I. Katernuk is grateful to the German–Russian Interdisciplinary Science Center (G-RISC) for the supporting one-month internship (project C-2019a-2) at the Institute of Technical Chemistry, Leibniz University Hannover (Hannover, Germany).
PY - 2020/5/8
Y1 - 2020/5/8
N2 - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.
AB - Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions-such as model oligonucleotide, siRNA, and pDNA-were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.
KW - Heparin
KW - Interpolyelectrolyte complex
KW - PDNA
KW - Photosensitive
KW - Poly-l-lysine
KW - SiRNA
UR - http://www.scopus.com/inward/record.url?scp=85085328279&partnerID=8YFLogxK
U2 - 10.3390/POLYM12051077
DO - 10.3390/POLYM12051077
M3 - Article
C2 - 32397208
AN - SCOPUS:85085328279
VL - 12
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 5
M1 - 1077
ER -