Details
| Original language | English |
|---|---|
| Pages (from-to) | 147-154 |
| Number of pages | 8 |
| Journal | Biotechnology and Applied Biochemistry |
| Volume | 60 |
| Issue number | 2 |
| Publication status | Published - 18 Apr 2013 |
Abstract
The pfah2 gene coding for a novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified during sequencing of random clones from a cDNA library. The corresponding protein sequence of PfaH2 deduced from the cDNA comprised 134 amino acids (aa). A 16 aa signal sequence preceded the N-terminus of the mature protein. PfaH2 belonged to the class Ia hydrophobins. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. The surface activity of the recently described PfaH1 and of PfaH2 was compared by the determination of contact angles (CAs) on glass slides and Teflon tape, and the CA of distilled water droplets was measured on glass slides coated with hydrophobin PfaH1 or PfaH2. Surprisingly, both hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to a similar quantitative extent, although little aa sequence homology was found.
Keywords
- ascomycete, contact angle, fungi, hydropathy plot, hydrophobin, Paecilomyces farinosus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Engineering(all)
- Biomedical Engineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Drug Discovery
- Chemical Engineering(all)
- Process Chemistry and Technology
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In: Biotechnology and Applied Biochemistry, Vol. 60, No. 2, 18.04.2013, p. 147-154.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - PfaH2
T2 - A novel hydrophobin from the ascomycete Paecilomyces farinosus
AU - Zelena, Katerina
AU - Takenberg, Meike
AU - Lunkenbein, Stefan
AU - Woche, Susanne K.
AU - Nimtz, Manfred
AU - Berger, Ralf G.
PY - 2013/4/18
Y1 - 2013/4/18
N2 - The pfah2 gene coding for a novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified during sequencing of random clones from a cDNA library. The corresponding protein sequence of PfaH2 deduced from the cDNA comprised 134 amino acids (aa). A 16 aa signal sequence preceded the N-terminus of the mature protein. PfaH2 belonged to the class Ia hydrophobins. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. The surface activity of the recently described PfaH1 and of PfaH2 was compared by the determination of contact angles (CAs) on glass slides and Teflon tape, and the CA of distilled water droplets was measured on glass slides coated with hydrophobin PfaH1 or PfaH2. Surprisingly, both hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to a similar quantitative extent, although little aa sequence homology was found.
AB - The pfah2 gene coding for a novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified during sequencing of random clones from a cDNA library. The corresponding protein sequence of PfaH2 deduced from the cDNA comprised 134 amino acids (aa). A 16 aa signal sequence preceded the N-terminus of the mature protein. PfaH2 belonged to the class Ia hydrophobins. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. The surface activity of the recently described PfaH1 and of PfaH2 was compared by the determination of contact angles (CAs) on glass slides and Teflon tape, and the CA of distilled water droplets was measured on glass slides coated with hydrophobin PfaH1 or PfaH2. Surprisingly, both hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to a similar quantitative extent, although little aa sequence homology was found.
KW - ascomycete
KW - contact angle
KW - fungi
KW - hydropathy plot
KW - hydrophobin
KW - Paecilomyces farinosus
UR - http://www.scopus.com/inward/record.url?scp=84876494780&partnerID=8YFLogxK
U2 - 10.1002/bab.1077
DO - 10.1002/bab.1077
M3 - Article
C2 - 23600571
AN - SCOPUS:84876494780
VL - 60
SP - 147
EP - 154
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
SN - 0885-4513
IS - 2
ER -