Details
| Original language | English |
|---|---|
| Title of host publication | Frontiers in Ultrafast Optics |
| Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XV |
| Editors | Michel Meunier, Peter R. Herman, Stefan Nolte, Alexander Heisterkamp |
| Publisher | SPIE |
| ISBN (electronic) | 9781628414455 |
| Publication status | Published - 9 Mar 2015 |
| Externally published | Yes |
| Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV - San Francisco, United States Duration: 8 Feb 2015 → 10 Feb 2015 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 9355 |
| ISSN (Print) | 0277-786X |
| ISSN (electronic) | 1996-756X |
Abstract
The interaction of cell-adhered nanostructures with laser light has attracted much interest within the biomedical field. Molecular delivery using a variety of plasmonic nanostructures, such as structured surfaces, nanoparticles and particle clusters, is currently evolving from its proof-of-concept into a routine method. Here, gold represents the material of choice, as it provides unique optical properties, different surface modifications as well as biocompatibility. In addition, new materials (e.g. polypyrrole) provide interesting alternatives. Applying this approach, a variety of molecules, such as fluorescent dyes, proteins, antisense structures, and DNA, has been transfected in order to manipulate the cellular functions in different experimental settings. Antisense structures, for example, allow the efficient down regulation of the gene activity of a target, providing insights into the gene's function. The delivery of proteins, as executing molecules in the cell, can exhibit an immediate effect on the cell behavior, allowing a minute observation of the intracellular kinetics. Direct cell manipulation can be achieved with this approach as well. Increasing the nanoparticle concentration and/or the radiant exposure, effective cell destruction is induced. Using targeted nanoparticles (e.g. by antibody conjugation) in combination with spatially selective laser irradiation permits well-directed cell manipulation even in mixed cultures and potentially in tissues. Furthermore, excited gold nanoparticles can directly trigger cellular reactions, which can possibly be utilized for cell stimulation. The manifold possibilities of nanostructure assisted laser manipulation are still in development.
Keywords
- antisense, Laser transfection, nanostructures, plasmonics
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV. ed. / Michel Meunier; Peter R. Herman; Stefan Nolte; Alexander Heisterkamp. SPIE, 2015. 935509 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9355).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Perspectives in nanostructure assisted laser manipulation of mammalian cells
AU - Heinemann, Dag
AU - Schomaker, Markus
AU - Kalies, Stefan
AU - Hoerdt, Anton
AU - Murua Escobar, Hugo
AU - Ripken, Tammo
AU - Meyer, Heiko
PY - 2015/3/9
Y1 - 2015/3/9
N2 - The interaction of cell-adhered nanostructures with laser light has attracted much interest within the biomedical field. Molecular delivery using a variety of plasmonic nanostructures, such as structured surfaces, nanoparticles and particle clusters, is currently evolving from its proof-of-concept into a routine method. Here, gold represents the material of choice, as it provides unique optical properties, different surface modifications as well as biocompatibility. In addition, new materials (e.g. polypyrrole) provide interesting alternatives. Applying this approach, a variety of molecules, such as fluorescent dyes, proteins, antisense structures, and DNA, has been transfected in order to manipulate the cellular functions in different experimental settings. Antisense structures, for example, allow the efficient down regulation of the gene activity of a target, providing insights into the gene's function. The delivery of proteins, as executing molecules in the cell, can exhibit an immediate effect on the cell behavior, allowing a minute observation of the intracellular kinetics. Direct cell manipulation can be achieved with this approach as well. Increasing the nanoparticle concentration and/or the radiant exposure, effective cell destruction is induced. Using targeted nanoparticles (e.g. by antibody conjugation) in combination with spatially selective laser irradiation permits well-directed cell manipulation even in mixed cultures and potentially in tissues. Furthermore, excited gold nanoparticles can directly trigger cellular reactions, which can possibly be utilized for cell stimulation. The manifold possibilities of nanostructure assisted laser manipulation are still in development.
AB - The interaction of cell-adhered nanostructures with laser light has attracted much interest within the biomedical field. Molecular delivery using a variety of plasmonic nanostructures, such as structured surfaces, nanoparticles and particle clusters, is currently evolving from its proof-of-concept into a routine method. Here, gold represents the material of choice, as it provides unique optical properties, different surface modifications as well as biocompatibility. In addition, new materials (e.g. polypyrrole) provide interesting alternatives. Applying this approach, a variety of molecules, such as fluorescent dyes, proteins, antisense structures, and DNA, has been transfected in order to manipulate the cellular functions in different experimental settings. Antisense structures, for example, allow the efficient down regulation of the gene activity of a target, providing insights into the gene's function. The delivery of proteins, as executing molecules in the cell, can exhibit an immediate effect on the cell behavior, allowing a minute observation of the intracellular kinetics. Direct cell manipulation can be achieved with this approach as well. Increasing the nanoparticle concentration and/or the radiant exposure, effective cell destruction is induced. Using targeted nanoparticles (e.g. by antibody conjugation) in combination with spatially selective laser irradiation permits well-directed cell manipulation even in mixed cultures and potentially in tissues. Furthermore, excited gold nanoparticles can directly trigger cellular reactions, which can possibly be utilized for cell stimulation. The manifold possibilities of nanostructure assisted laser manipulation are still in development.
KW - antisense
KW - Laser transfection
KW - nanostructures
KW - plasmonics
UR - http://www.scopus.com/inward/record.url?scp=84930074955&partnerID=8YFLogxK
U2 - 10.1117/12.2086679
DO - 10.1117/12.2086679
M3 - Conference contribution
AN - SCOPUS:84930074955
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
A2 - Meunier, Michel
A2 - Herman, Peter R.
A2 - Nolte, Stefan
A2 - Heisterkamp, Alexander
PB - SPIE
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV
Y2 - 8 February 2015 through 10 February 2015
ER -