Details
| Original language | English |
|---|---|
| Pages (from-to) | 7011-7020 |
| Number of pages | 10 |
| Journal | ACS Applied Bio Materials |
| Volume | 3 |
| Issue number | 10 |
| Early online date | 17 Sept 2020 |
| Publication status | Published - 19 Oct 2020 |
Abstract
Hydrogels are favored materials in tissue engineering as they can be used to imitate tissues, provide scaffolds, and guide cell behavior. Recent advances in the field of optogenetics have created a need for biocompatible optical waveguides, and hydrogels have been investigated to meet these requirements. However, combining favorable waveguiding characteristics, high biocompatibility, and controllable bioactivity in a single device remains challenging. Here, we investigate the use of poly(ethylene glycol) hydrogels as carriers and illumination systems for in vitro cell culture. We present a comprehensive and reproducible protocol for selective bioactivation of the hydrogels, achieving high proliferation rates and strong cell adhesion on the treated surface. A cell model expressing the photoconvertible fluorescent protein Dendra2 confirmed that light-cell interactions occur at the hydrogel surface. Monte Carlo simulations were performed as a tool to predict the extent of these interactions. This study demonstrates a hydrogel-based waveguiding system for targeted cell stimulation in vitro and potentially in vivo environments.
Keywords
- cell adhesion, hydrogel scaffold, Monte Carlo simulation, PEGDMA hydrogels, photoconversion, waveguides
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Medicine(all)
- Biochemistry, medical
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In: ACS Applied Bio Materials, Vol. 3, No. 10, 19.10.2020, p. 7011-7020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - PEGDMA Hydrogels for Cell Adhesion and Optical Waveguiding
AU - Johannsmeier, Sonja
AU - Nguyen, Minh Thanh Truc
AU - Hohndorf, Ruben
AU - Dräger, Gerald
AU - Heinemann, Dag
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
N1 - Funding information: This work was funded by the Federal Ministry of Education and Research, Germany, within the funding program Photonics Research Germany, project BioPACE (13N14085).
PY - 2020/10/19
Y1 - 2020/10/19
N2 - Hydrogels are favored materials in tissue engineering as they can be used to imitate tissues, provide scaffolds, and guide cell behavior. Recent advances in the field of optogenetics have created a need for biocompatible optical waveguides, and hydrogels have been investigated to meet these requirements. However, combining favorable waveguiding characteristics, high biocompatibility, and controllable bioactivity in a single device remains challenging. Here, we investigate the use of poly(ethylene glycol) hydrogels as carriers and illumination systems for in vitro cell culture. We present a comprehensive and reproducible protocol for selective bioactivation of the hydrogels, achieving high proliferation rates and strong cell adhesion on the treated surface. A cell model expressing the photoconvertible fluorescent protein Dendra2 confirmed that light-cell interactions occur at the hydrogel surface. Monte Carlo simulations were performed as a tool to predict the extent of these interactions. This study demonstrates a hydrogel-based waveguiding system for targeted cell stimulation in vitro and potentially in vivo environments.
AB - Hydrogels are favored materials in tissue engineering as they can be used to imitate tissues, provide scaffolds, and guide cell behavior. Recent advances in the field of optogenetics have created a need for biocompatible optical waveguides, and hydrogels have been investigated to meet these requirements. However, combining favorable waveguiding characteristics, high biocompatibility, and controllable bioactivity in a single device remains challenging. Here, we investigate the use of poly(ethylene glycol) hydrogels as carriers and illumination systems for in vitro cell culture. We present a comprehensive and reproducible protocol for selective bioactivation of the hydrogels, achieving high proliferation rates and strong cell adhesion on the treated surface. A cell model expressing the photoconvertible fluorescent protein Dendra2 confirmed that light-cell interactions occur at the hydrogel surface. Monte Carlo simulations were performed as a tool to predict the extent of these interactions. This study demonstrates a hydrogel-based waveguiding system for targeted cell stimulation in vitro and potentially in vivo environments.
KW - cell adhesion
KW - hydrogel scaffold
KW - Monte Carlo simulation
KW - PEGDMA hydrogels
KW - photoconversion
KW - waveguides
UR - http://www.scopus.com/inward/record.url?scp=85096493642&partnerID=8YFLogxK
U2 - 10.1021/acsabm.0c00885
DO - 10.1021/acsabm.0c00885
M3 - Article
AN - SCOPUS:85096493642
VL - 3
SP - 7011
EP - 7020
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 10
ER -