Details
| Original language | English |
|---|---|
| Pages (from-to) | 153-166 |
| Number of pages | 14 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 126 |
| Issue number | 1 |
| Early online date | 1 Apr 2016 |
| Publication status | Published - Jul 2016 |
Abstract
This study provides a basic tool for the production of a multiclonal variety of Juglans nigra × J. regia hybrids (J. × intermedia), including optimized in vitro propagation methods and DNA-based techniques for the identification of hybrid seeds. Following identification of hybrid seed material using DNA markers embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of shoot cultures of four hybrid genotypes was compared on two different basal media (Rugini and DKW) with three concentrations (2.2; 4.4; and 8.8 µM) of 6-benzylaminopurine (BA), and three gelling agents (Oxoid agar, Kobe agar, Gelrite). Three indole-3-butyric acid (IBA) concentrations (4, 12, and 20 µM) were compared for root induction, as well as three media for root expression. In general, the best combination of shoot elongation and production of new axillary shoots was achieved with 4.4 μM BA and 0.2 μM IBA in Rugini and in DKW medium. However, shoot elongation in most genotypes was favored when 2.2 μM BA and 0.2 μM IBA was used, in both, DKW and Rugini medium. The optimal gelling agent for Juglans hybrid shoot cultures was Kobe agar in Rugini medium. Highest rooting percentages were obtained on DKW medium with 12 µM IBA with 5 days in darkness followed by root expression under light on a mixture of gelified ¼ DKW medium and vermiculite. During acclimatization, more than 75 % of the plantlets continued to grow vigorously.
Keywords
- DNA fingerprinting, In vitro propagation, Juglans hybrids, Rooting, Rugini medium, Shoot proliferation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 126, No. 1, 07.2016, p. 153-166.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Paving the way for large-scale micropropagation of Juglans × intermedia using genetically identified hybrid seed
AU - Tuan, P.N.
AU - Meier-Dinkel, A.
AU - Höltken, A.M.
AU - Wenzlitschke, I.
AU - Winkelmann, T.
N1 - We thank the Government of Vietnam and NW-FVA (Northwest German Forest Research Institute) for Funding and providing facilities. We also thank Dr. Nguyen Van Thinh for editing the manuscript.
PY - 2016/7
Y1 - 2016/7
N2 - This study provides a basic tool for the production of a multiclonal variety of Juglans nigra × J. regia hybrids (J. × intermedia), including optimized in vitro propagation methods and DNA-based techniques for the identification of hybrid seeds. Following identification of hybrid seed material using DNA markers embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of shoot cultures of four hybrid genotypes was compared on two different basal media (Rugini and DKW) with three concentrations (2.2; 4.4; and 8.8 µM) of 6-benzylaminopurine (BA), and three gelling agents (Oxoid agar, Kobe agar, Gelrite). Three indole-3-butyric acid (IBA) concentrations (4, 12, and 20 µM) were compared for root induction, as well as three media for root expression. In general, the best combination of shoot elongation and production of new axillary shoots was achieved with 4.4 μM BA and 0.2 μM IBA in Rugini and in DKW medium. However, shoot elongation in most genotypes was favored when 2.2 μM BA and 0.2 μM IBA was used, in both, DKW and Rugini medium. The optimal gelling agent for Juglans hybrid shoot cultures was Kobe agar in Rugini medium. Highest rooting percentages were obtained on DKW medium with 12 µM IBA with 5 days in darkness followed by root expression under light on a mixture of gelified ¼ DKW medium and vermiculite. During acclimatization, more than 75 % of the plantlets continued to grow vigorously.
AB - This study provides a basic tool for the production of a multiclonal variety of Juglans nigra × J. regia hybrids (J. × intermedia), including optimized in vitro propagation methods and DNA-based techniques for the identification of hybrid seeds. Following identification of hybrid seed material using DNA markers embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of shoot cultures of four hybrid genotypes was compared on two different basal media (Rugini and DKW) with three concentrations (2.2; 4.4; and 8.8 µM) of 6-benzylaminopurine (BA), and three gelling agents (Oxoid agar, Kobe agar, Gelrite). Three indole-3-butyric acid (IBA) concentrations (4, 12, and 20 µM) were compared for root induction, as well as three media for root expression. In general, the best combination of shoot elongation and production of new axillary shoots was achieved with 4.4 μM BA and 0.2 μM IBA in Rugini and in DKW medium. However, shoot elongation in most genotypes was favored when 2.2 μM BA and 0.2 μM IBA was used, in both, DKW and Rugini medium. The optimal gelling agent for Juglans hybrid shoot cultures was Kobe agar in Rugini medium. Highest rooting percentages were obtained on DKW medium with 12 µM IBA with 5 days in darkness followed by root expression under light on a mixture of gelified ¼ DKW medium and vermiculite. During acclimatization, more than 75 % of the plantlets continued to grow vigorously.
KW - DNA fingerprinting
KW - In vitro propagation
KW - Juglans hybrids
KW - Rooting
KW - Rugini medium
KW - Shoot proliferation
UR - http://www.scopus.com/inward/record.url?scp=84962030726&partnerID=8YFLogxK
U2 - 10.1007/s11240-016-0986-5
DO - 10.1007/s11240-016-0986-5
M3 - Article
VL - 126
SP - 153
EP - 166
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -