Details
| Original language | English |
|---|---|
| Pages (from-to) | 9218-9230 |
| Number of pages | 13 |
| Journal | BIOMATERIALS |
| Volume | 32 |
| Issue number | 35 |
| Publication status | Published - 10 Sept 2011 |
| Externally published | Yes |
Abstract
Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
Keywords
- Cardiac patch, Cardiac regeneration, Human mesenchymal stem cells, Human umbilical vein endothelial cells, Laser-induced forward transfer cell printing
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Ceramics and Composites
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Mechanics of Materials
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In: BIOMATERIALS, Vol. 32, No. 35, 10.09.2011, p. 9218-9230.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration
AU - Gaebel, Ralf
AU - Ma, Nan
AU - Liu, Jun
AU - Guan, Jianjun
AU - Koch, Lothar
AU - Klopsch, Christian
AU - Gruene, Martin
AU - Toelk, Anita
AU - Wang, Weiwei
AU - Mark, Peter
AU - Wang, Feng
AU - Chichkov, Boris
AU - Li, Wenzhong
AU - Steinhoff, Gustav
N1 - Funding information: This work was supported by Standardization for Regenerative Therapy - Mesenchymal Stem Cells (START-MSC) ; Sonderforschungsbereich/Transregio 37 (B5, B2 and A4); German Federal Ministry of Education and Research , BioChancePlus program (0313191); The German Helmholtz Association , Mecklenburg-Vorpommern , German Federal Ministry of Education and Research , German Research Foundation (Nachwuchsgruppe Regenerative Medizin Regulation der Stammzellmigration 0402710); REBIRTH Cluster of Excellence (Exc62/1) ; Förderkennzeichen 0312138 A (Ministry of Education (Germany, Berlin)); V220-630-08-TFMV-F/S-035 (Ministry of Economy (Mecklenburg-West Pommerania, Schwerin)); Marie Curie International Research Staff Exchange Scheme (IRSES, FP7-PEOPLE-2009-IRSES); the Reference and Translation Center for Cardiac Stem Cell Therapy (RTC) .
PY - 2011/9/10
Y1 - 2011/9/10
N2 - Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
AB - Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
KW - Cardiac patch
KW - Cardiac regeneration
KW - Human mesenchymal stem cells
KW - Human umbilical vein endothelial cells
KW - Laser-induced forward transfer cell printing
UR - http://www.scopus.com/inward/record.url?scp=80053604735&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2011.08.071
DO - 10.1016/j.biomaterials.2011.08.071
M3 - Article
C2 - 21911255
AN - SCOPUS:80053604735
VL - 32
SP - 9218
EP - 9230
JO - BIOMATERIALS
JF - BIOMATERIALS
SN - 0142-9612
IS - 35
ER -