Details
| Original language | English |
|---|---|
| Pages (from-to) | 838-846 |
| Number of pages | 9 |
| Journal | Plant physiology and biochemistry |
| Volume | 47 |
| Issue number | 9 |
| Publication status | Published - 19 May 2009 |
Abstract
A comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (Solanum lycopersicum) to infection with Ralstonia solanacearum, causal agent of bacterial wilt. Plants of the susceptible tomato recombinant inbred line NHG3 and the resistant NHG13 were either infected or not infected with R. solanacearum and subsequently used for proteome analysis. Two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-D IEF/SDS-PAGE) allowed the separation of about 650-690 protein spots per analysis. Twelve proteins were of differential abundance in susceptible plants in response to bacterial infection, while no differences were observed in the resistant genotype. LC-MS/MS analysis of these spots revealed 12 proteins, six of which were annotated as plant and six as bacterial proteins. Among the plant proteins, two represent pathogenesis related (PR) proteins, one stress response protein, one enzyme of carbohydrate and energy metabolism, and one hypothetical protein. A constitutive difference between resistant and susceptible lines was not found.
Keywords
- 2-D gel electrophoresis, Bacterial wilt, LC-MS/MS, PR protein, Ralstonia solanacearum, Solanum lycopersicum
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant physiology and biochemistry, Vol. 47, No. 9, 19.05.2009, p. 838-846.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Pathogenesis and stress related, as well as metabolic proteins are regulated in tomato stems infected with Ralstonia solanacearum
AU - Dahal, Diwakar
AU - Heintz, Dimitri
AU - Van Dorsselaer, Alain
AU - Braun, Hans Peter
AU - Wydra, Kerstin
N1 - Funding information: This study was funded by the Federal Ministry for Economic Cooperation and Development (BMZ), Germany, in the framework of the collaborative project, project no. 03.7860.4-001.00 with the Asian Vegetable Research and Development Center (AVRDC), Taiwan.
PY - 2009/5/19
Y1 - 2009/5/19
N2 - A comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (Solanum lycopersicum) to infection with Ralstonia solanacearum, causal agent of bacterial wilt. Plants of the susceptible tomato recombinant inbred line NHG3 and the resistant NHG13 were either infected or not infected with R. solanacearum and subsequently used for proteome analysis. Two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-D IEF/SDS-PAGE) allowed the separation of about 650-690 protein spots per analysis. Twelve proteins were of differential abundance in susceptible plants in response to bacterial infection, while no differences were observed in the resistant genotype. LC-MS/MS analysis of these spots revealed 12 proteins, six of which were annotated as plant and six as bacterial proteins. Among the plant proteins, two represent pathogenesis related (PR) proteins, one stress response protein, one enzyme of carbohydrate and energy metabolism, and one hypothetical protein. A constitutive difference between resistant and susceptible lines was not found.
AB - A comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (Solanum lycopersicum) to infection with Ralstonia solanacearum, causal agent of bacterial wilt. Plants of the susceptible tomato recombinant inbred line NHG3 and the resistant NHG13 were either infected or not infected with R. solanacearum and subsequently used for proteome analysis. Two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-D IEF/SDS-PAGE) allowed the separation of about 650-690 protein spots per analysis. Twelve proteins were of differential abundance in susceptible plants in response to bacterial infection, while no differences were observed in the resistant genotype. LC-MS/MS analysis of these spots revealed 12 proteins, six of which were annotated as plant and six as bacterial proteins. Among the plant proteins, two represent pathogenesis related (PR) proteins, one stress response protein, one enzyme of carbohydrate and energy metabolism, and one hypothetical protein. A constitutive difference between resistant and susceptible lines was not found.
KW - 2-D gel electrophoresis
KW - Bacterial wilt
KW - LC-MS/MS
KW - PR protein
KW - Ralstonia solanacearum
KW - Solanum lycopersicum
UR - http://www.scopus.com/inward/record.url?scp=67649484363&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2009.05.001
DO - 10.1016/j.plaphy.2009.05.001
M3 - Article
C2 - 19482482
AN - SCOPUS:67649484363
VL - 47
SP - 838
EP - 846
JO - Plant physiology and biochemistry
JF - Plant physiology and biochemistry
SN - 0981-9428
IS - 9
ER -