Details
| Original language | English |
|---|---|
| Pages (from-to) | 335-345 |
| Number of pages | 11 |
| Journal | New Phytologist |
| Volume | 195 |
| Issue number | 2 |
| Publication status | Published - Jul 2012 |
Abstract
• Floral scent is a complex trait of biological and applied significance. To evaluate whether scent production originating from diverse metabolic pathways (e.g. phenylpropanoids and isoprenoids) can be affected by transcriptional regulators, Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1) transcription factor was introduced into Rosa hybrida. • Color and scent profiles of PAP1-transgenic and control (β-glucuronidase-expressing) rose flowers and the expression of key genes involved in the production of secondary metabolites were analyzed. To evaluate the significance of the scent modification, olfactory trials were conducted with both humans and honeybees. • In addition to increased levels of phenylpropanoid-derived color and scent compounds when compared with control flowers, PAP1-transgenic rose lines also emitted up to 6.5 times higher levels of terpenoid scent compounds. Olfactory assay revealed that bees and humans could discriminate between the floral scents of PAP1-transgenic and control flowers. • The increase in volatile production in PAP1 transgenes was not caused solely by transcriptional activation of their respective biosynthetic genes, but probably also resulted from enhanced metabolic flux in both the phenylpropanoid and isoprenoid pathways. The mechanism(s) governing the interactions in these metabolic pathways that are responsible for the production of specialized metabolites remains to be elucidated.
Keywords
- Olfactory, Phenylpropanoid, PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1), Rosa hybrida, Scent, Terpenoid
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: New Phytologist, Vol. 195, No. 2, 07.2012, p. 335-345.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers
AU - Zvi, Michal Moyal Ben
AU - Shklarman, Elena
AU - Masci, Tania
AU - Kalev, Haim
AU - Debener, Thomas
AU - Shafir, Sharoni
AU - Ovadis, Marianna
AU - Vainstein, Alexander
PY - 2012/7
Y1 - 2012/7
N2 - • Floral scent is a complex trait of biological and applied significance. To evaluate whether scent production originating from diverse metabolic pathways (e.g. phenylpropanoids and isoprenoids) can be affected by transcriptional regulators, Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1) transcription factor was introduced into Rosa hybrida. • Color and scent profiles of PAP1-transgenic and control (β-glucuronidase-expressing) rose flowers and the expression of key genes involved in the production of secondary metabolites were analyzed. To evaluate the significance of the scent modification, olfactory trials were conducted with both humans and honeybees. • In addition to increased levels of phenylpropanoid-derived color and scent compounds when compared with control flowers, PAP1-transgenic rose lines also emitted up to 6.5 times higher levels of terpenoid scent compounds. Olfactory assay revealed that bees and humans could discriminate between the floral scents of PAP1-transgenic and control flowers. • The increase in volatile production in PAP1 transgenes was not caused solely by transcriptional activation of their respective biosynthetic genes, but probably also resulted from enhanced metabolic flux in both the phenylpropanoid and isoprenoid pathways. The mechanism(s) governing the interactions in these metabolic pathways that are responsible for the production of specialized metabolites remains to be elucidated.
AB - • Floral scent is a complex trait of biological and applied significance. To evaluate whether scent production originating from diverse metabolic pathways (e.g. phenylpropanoids and isoprenoids) can be affected by transcriptional regulators, Arabidopsis PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1) transcription factor was introduced into Rosa hybrida. • Color and scent profiles of PAP1-transgenic and control (β-glucuronidase-expressing) rose flowers and the expression of key genes involved in the production of secondary metabolites were analyzed. To evaluate the significance of the scent modification, olfactory trials were conducted with both humans and honeybees. • In addition to increased levels of phenylpropanoid-derived color and scent compounds when compared with control flowers, PAP1-transgenic rose lines also emitted up to 6.5 times higher levels of terpenoid scent compounds. Olfactory assay revealed that bees and humans could discriminate between the floral scents of PAP1-transgenic and control flowers. • The increase in volatile production in PAP1 transgenes was not caused solely by transcriptional activation of their respective biosynthetic genes, but probably also resulted from enhanced metabolic flux in both the phenylpropanoid and isoprenoid pathways. The mechanism(s) governing the interactions in these metabolic pathways that are responsible for the production of specialized metabolites remains to be elucidated.
KW - Olfactory
KW - Phenylpropanoid
KW - PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1)
KW - Rosa hybrida
KW - Scent
KW - Terpenoid
UR - http://www.scopus.com/inward/record.url?scp=84862570447&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.2012.04161.x
DO - 10.1111/j.1469-8137.2012.04161.x
M3 - Article
C2 - 22548501
AN - SCOPUS:84862570447
VL - 195
SP - 335
EP - 345
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 2
ER -