Details
| Original language | English |
|---|---|
| Pages (from-to) | 545-552 |
| Number of pages | 8 |
| Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Volume | 49 |
| Issue number | 9-10 |
| Publication status | Published - Oct 1994 |
Abstract
Several strains of basidiomycetes were examined for their ability to transform ɑ- and (ß-pinene in agitated submerged, cultures. Four major metabolites of ß-pinene (verbenol, verbenone, myrtenol, and trans-pinocarveol) and three main metabolites of (ß-pinene (1,4-cineol, myrtenol, and trans-pinocarveol) were isolated from the fermentation, broth. The metabolic pathways included allylic oxidation, oxidative cleavage and further regioselective, oxidation. Ganoderma applanatum was found to carry out the stereoselective allylic hydroxylation of ß-pinene to verbenol, and of (ß-pinene to trans-pinocarveol in trans position to the C-C, bridge. The optimal conditions of the bioreaction were established with respect to substrate concentration, incubation time and conversion, time. Due to growth inhibition caused by elevated substrate concentration, the bioconversion of (ß-pinene required pre-grown, cultures. Generally, mycelial pellet cultures were supplemented with the terpene substrate when a residual glucose content of 50% was, reached. Depending on strain this point was reached after about 48 h. An incubation period of two to three days gave best, yields. The transient accumulation of oxygenated products apparently reflected different reaction velocities of the successive catabolic, steps.
Keywords
- Basidiomycetes, Bioconversion, Cineol, Pinene, Transformation
ASJC Scopus subject areas
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In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 49, No. 9-10, 10.1994, p. 545-552.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Oxyfunctionalization of ɑ- and β-Pinene by Selected Basidiomycetes
AU - Busmann, Dagmar
AU - Berger, Ralf Günter
N1 - Funding information: This work was supported in part by the DFG (Deutsche Forschungsgemeinschaft) via the Graduiertenkolleg “Naturstofftransformation”.
PY - 1994/10
Y1 - 1994/10
N2 - Several strains of basidiomycetes were examined for their ability to transform ɑ- and (ß-pinene in agitated submerged, cultures. Four major metabolites of ß-pinene (verbenol, verbenone, myrtenol, and trans-pinocarveol) and three main metabolites of (ß-pinene (1,4-cineol, myrtenol, and trans-pinocarveol) were isolated from the fermentation, broth. The metabolic pathways included allylic oxidation, oxidative cleavage and further regioselective, oxidation. Ganoderma applanatum was found to carry out the stereoselective allylic hydroxylation of ß-pinene to verbenol, and of (ß-pinene to trans-pinocarveol in trans position to the C-C, bridge. The optimal conditions of the bioreaction were established with respect to substrate concentration, incubation time and conversion, time. Due to growth inhibition caused by elevated substrate concentration, the bioconversion of (ß-pinene required pre-grown, cultures. Generally, mycelial pellet cultures were supplemented with the terpene substrate when a residual glucose content of 50% was, reached. Depending on strain this point was reached after about 48 h. An incubation period of two to three days gave best, yields. The transient accumulation of oxygenated products apparently reflected different reaction velocities of the successive catabolic, steps.
AB - Several strains of basidiomycetes were examined for their ability to transform ɑ- and (ß-pinene in agitated submerged, cultures. Four major metabolites of ß-pinene (verbenol, verbenone, myrtenol, and trans-pinocarveol) and three main metabolites of (ß-pinene (1,4-cineol, myrtenol, and trans-pinocarveol) were isolated from the fermentation, broth. The metabolic pathways included allylic oxidation, oxidative cleavage and further regioselective, oxidation. Ganoderma applanatum was found to carry out the stereoselective allylic hydroxylation of ß-pinene to verbenol, and of (ß-pinene to trans-pinocarveol in trans position to the C-C, bridge. The optimal conditions of the bioreaction were established with respect to substrate concentration, incubation time and conversion, time. Due to growth inhibition caused by elevated substrate concentration, the bioconversion of (ß-pinene required pre-grown, cultures. Generally, mycelial pellet cultures were supplemented with the terpene substrate when a residual glucose content of 50% was, reached. Depending on strain this point was reached after about 48 h. An incubation period of two to three days gave best, yields. The transient accumulation of oxygenated products apparently reflected different reaction velocities of the successive catabolic, steps.
KW - Basidiomycetes
KW - Bioconversion
KW - Cineol
KW - Pinene
KW - Transformation
UR - http://www.scopus.com/inward/record.url?scp=0028152168&partnerID=8YFLogxK
U2 - 10.1515/znc-1994-9-1003
DO - 10.1515/znc-1994-9-1003
M3 - Article
AN - SCOPUS:0028152168
VL - 49
SP - 545
EP - 552
JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
SN - 0939-5075
IS - 9-10
ER -