Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Publisher | International Society for Horticultural Science |
| Pages | 195-200 |
| Number of pages | 6 |
| ISBN (print) | 9789066051102 |
| Publication status | Published - 28 Feb 2010 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 855 |
| ISSN (Print) | 0567-7572 |
Abstract
In order to establish protocols for embryo rescue techniques, in a first step this study aimed at the identification of culture conditions for ovules from intraspecific crosses. The effects of dissection date after pollination and temperature during ovule culture were examined in intraspecific crossings of Helleborus niger, H. argutifolius, H. × hybridus and H. foetidus. Ovaries were harvested 3-6 weeks after pollination, surface disinfected in 70% ethanol for 30 s, 2% sodium hypochlorite with one drop Tween for 10 min and rinsed in sterilised water three times. Ovules were dissected from ovaries and cultured on medium based on MS solidified with 0.4% Gelrite at a pH of 5.8. Two media supplemented with 2.5 or 5% sucrose were compared. Ovules were cultured in darkness at 24±1 or 16±1°C for 12 weeks. Thereafter, ovules of each temperature treatment were split and one half was incubated at 6±1°C for 11 weeks, while the other half remained in the initial temperature. Afterwards the ovules were placed back to their initial temperature. On average of all species, 2% of the ovules dissected 3 weeks and 1% dissected 4 weeks after pollination germinated, while preparation after 5 weeks resulted in 4% and after 6 weeks in 8% germination, respectively. The intermediate cold treatment with 6°C turned out to be very beneficial for later germination. Furthermore, no effect of the media supplemented with either 2.5 or 5% sucrose was observed.
Keywords
- Germination, Helleborus, Ovule culture
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. International Society for Horticultural Science, 2010. p. 195-200 (Acta Horticulturae; Vol. 855).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Ovule culture of Helleborus species
AU - Meiners, J.
AU - Winkelmann, T.
PY - 2010/2/28
Y1 - 2010/2/28
N2 - In order to establish protocols for embryo rescue techniques, in a first step this study aimed at the identification of culture conditions for ovules from intraspecific crosses. The effects of dissection date after pollination and temperature during ovule culture were examined in intraspecific crossings of Helleborus niger, H. argutifolius, H. × hybridus and H. foetidus. Ovaries were harvested 3-6 weeks after pollination, surface disinfected in 70% ethanol for 30 s, 2% sodium hypochlorite with one drop Tween for 10 min and rinsed in sterilised water three times. Ovules were dissected from ovaries and cultured on medium based on MS solidified with 0.4% Gelrite at a pH of 5.8. Two media supplemented with 2.5 or 5% sucrose were compared. Ovules were cultured in darkness at 24±1 or 16±1°C for 12 weeks. Thereafter, ovules of each temperature treatment were split and one half was incubated at 6±1°C for 11 weeks, while the other half remained in the initial temperature. Afterwards the ovules were placed back to their initial temperature. On average of all species, 2% of the ovules dissected 3 weeks and 1% dissected 4 weeks after pollination germinated, while preparation after 5 weeks resulted in 4% and after 6 weeks in 8% germination, respectively. The intermediate cold treatment with 6°C turned out to be very beneficial for later germination. Furthermore, no effect of the media supplemented with either 2.5 or 5% sucrose was observed.
AB - In order to establish protocols for embryo rescue techniques, in a first step this study aimed at the identification of culture conditions for ovules from intraspecific crosses. The effects of dissection date after pollination and temperature during ovule culture were examined in intraspecific crossings of Helleborus niger, H. argutifolius, H. × hybridus and H. foetidus. Ovaries were harvested 3-6 weeks after pollination, surface disinfected in 70% ethanol for 30 s, 2% sodium hypochlorite with one drop Tween for 10 min and rinsed in sterilised water three times. Ovules were dissected from ovaries and cultured on medium based on MS solidified with 0.4% Gelrite at a pH of 5.8. Two media supplemented with 2.5 or 5% sucrose were compared. Ovules were cultured in darkness at 24±1 or 16±1°C for 12 weeks. Thereafter, ovules of each temperature treatment were split and one half was incubated at 6±1°C for 11 weeks, while the other half remained in the initial temperature. Afterwards the ovules were placed back to their initial temperature. On average of all species, 2% of the ovules dissected 3 weeks and 1% dissected 4 weeks after pollination germinated, while preparation after 5 weeks resulted in 4% and after 6 weeks in 8% germination, respectively. The intermediate cold treatment with 6°C turned out to be very beneficial for later germination. Furthermore, no effect of the media supplemented with either 2.5 or 5% sucrose was observed.
KW - Germination
KW - Helleborus
KW - Ovule culture
UR - http://www.scopus.com/inward/record.url?scp=77952680669&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2010.855.28
DO - 10.17660/ActaHortic.2010.855.28
M3 - Conference contribution
AN - SCOPUS:77952680669
SN - 9789066051102
T3 - Acta Horticulturae
SP - 195
EP - 200
BT - Acta Horticulturae
PB - International Society for Horticultural Science
ER -