Details
| Original language | English |
|---|---|
| Pages (from-to) | 4917-4925 |
| Number of pages | 9 |
| Journal | Chemical science |
| Volume | 8 |
| Issue number | 7 |
| Publication status | Published - 1 Jan 2017 |
Abstract
Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.
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In: Chemical science, Vol. 8, No. 7, 01.01.2017, p. 4917-4925.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Orange, red, yellow
T2 - Biosynthesis of azaphilone pigments in Monascus fungi
AU - Chen, Wanping
AU - Chen, Runfa
AU - Liu, Qingpei
AU - He, Yi
AU - He, Kun
AU - Ding, Xiaoli
AU - Kang, Lijing
AU - Guo, Xiaoxiao
AU - Xie, Nana
AU - Zhou, Youxiang
AU - Lu, Yuanyuan
AU - Cox, Russell J.
AU - Molnár, István
AU - Li, Mu
AU - Shao, Yanchun
AU - Chen, Fusheng
N1 - Funding information: This study was supported by the Major Program of National Natural Science Foundation of China (No. 31330059 to F. C.), the National Natural Science Foundation of China (No. 31171649 to F. C., 31271834 to Y. S., 31371824 to Y. Z., 31401631 to M. L., and 31601446 to W. C.), the National Institutes of Health (NIGMS R01GM114418-01A1 to I. M.), and Deutsche Forschungsgemeinschaft (INST 187/621-1, LCMS equipment, to R. J. C). I. M. has a disclosed financial interest in TEVA Pharmaceuticals Hungary, which is unrelated to the subject of the research presented here. All the other authors declare no competing financial interests.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.
AB - Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.
UR - http://www.scopus.com/inward/record.url?scp=85021760170&partnerID=8YFLogxK
U2 - 10.1039/c7sc00475c
DO - 10.1039/c7sc00475c
M3 - Article
AN - SCOPUS:85021760170
VL - 8
SP - 4917
EP - 4925
JO - Chemical science
JF - Chemical science
SN - 2041-6520
IS - 7
ER -