Details
| Original language | English |
|---|---|
| Pages (from-to) | 207-215 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 540 |
| Issue number | C |
| Publication status | Published - 1991 |
Abstract
An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
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In: Journal of Chromatography A, Vol. 540, No. C, 1991, p. 207-215.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Optimized high-performance liquid chromatographic procedure for the separation and quantification of the main folacins and some derivatives. I. Chromatographic system
AU - Hahn, A.
AU - Stein, J.
AU - Rump, U.
AU - Rehner, G.
PY - 1991
Y1 - 1991
N2 - An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.
AB - An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.
UR - http://www.scopus.com/inward/record.url?scp=0026035112&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)88810-5
DO - 10.1016/S0021-9673(01)88810-5
M3 - Article
AN - SCOPUS:0026035112
VL - 540
SP - 207
EP - 215
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -