Details
| Original language | English |
|---|---|
| Number of pages | 16 |
| Journal | European Journal of Horticultural Science |
| Volume | 89 |
| Issue number | 2 |
| Publication status | Published - 22 May 2024 |
Abstract
Besides being an important oilseed crop, Brassica juncea is used as leafy vegetable, spice and biofumigation crop. To use novel breeding techniques such as genome editing, an efficient, reproducible regeneration protocol is beneficial. For B. juncea, adventitious shoot regeneration had been described previously, but mainly using lines of the Indian and Chinese gene pool. In this study, we aimed at establishing and improving an adventitious shoot regeneration protocol for current B. juncea cultivars and breeding lines from the European gene pool (‘Terraplus’, 'Terratop', ‘Terrafit’, 'Energy', SFB18/1 and SFB22/15) and compared their response to gene bank accessions originating from India. Cotyledon and hypocotyl explants of five-days-old seedlings were cultured on Murashige and Skoog medium supplemented with different concentrations BAP, IBA and NAA with or without 9.95 pM AgNO3. When the cotyledon and hypocotyl explants were cultured on media supplemented only with BAP and IBA, the regeneration rate was below 50% and 5%, respectively. The highest shoot regeneration rates of 58-72% and 65-90% for the European and Indian lines, respectively, as well as the highest shoot number of 2.7 and 3.0 per regenerating explant were obtained on a medium containing 8.88 pM BAP, 5.37 pM NAA and 9.95 pM AgNO3. Shoot regeneration rate was severely affected in all lines by ethylene production resulting from the addition of the ethylene precursor 1-aminocyclopropane-l-carboxylic acid to the medium. Also, AgNO3 caused an increased ethylene production measured after ten days of culture, but it had a significant positive effect on shoot regeneration indicating a negative effect of ethylene on early events in organogenesis.
Keywords
- Brassicaceae, brown mustard, ethylene, organogenesis, plant growth regulators, silver nitrate (AgNO)
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: European Journal of Horticultural Science, Vol. 89, No. 2, 22.05.2024.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Optimization of in vitro adventitious shoot regeneration in Brassica juncea L. of different origins for application in genetic transformation and genome editing
AU - Assou, J.
AU - Wamhoff, D.
AU - Rutzen, L.
AU - Winkelmann, T.
PY - 2024/5/22
Y1 - 2024/5/22
N2 - Besides being an important oilseed crop, Brassica juncea is used as leafy vegetable, spice and biofumigation crop. To use novel breeding techniques such as genome editing, an efficient, reproducible regeneration protocol is beneficial. For B. juncea, adventitious shoot regeneration had been described previously, but mainly using lines of the Indian and Chinese gene pool. In this study, we aimed at establishing and improving an adventitious shoot regeneration protocol for current B. juncea cultivars and breeding lines from the European gene pool (‘Terraplus’, 'Terratop', ‘Terrafit’, 'Energy', SFB18/1 and SFB22/15) and compared their response to gene bank accessions originating from India. Cotyledon and hypocotyl explants of five-days-old seedlings were cultured on Murashige and Skoog medium supplemented with different concentrations BAP, IBA and NAA with or without 9.95 pM AgNO3. When the cotyledon and hypocotyl explants were cultured on media supplemented only with BAP and IBA, the regeneration rate was below 50% and 5%, respectively. The highest shoot regeneration rates of 58-72% and 65-90% for the European and Indian lines, respectively, as well as the highest shoot number of 2.7 and 3.0 per regenerating explant were obtained on a medium containing 8.88 pM BAP, 5.37 pM NAA and 9.95 pM AgNO3. Shoot regeneration rate was severely affected in all lines by ethylene production resulting from the addition of the ethylene precursor 1-aminocyclopropane-l-carboxylic acid to the medium. Also, AgNO3 caused an increased ethylene production measured after ten days of culture, but it had a significant positive effect on shoot regeneration indicating a negative effect of ethylene on early events in organogenesis.
AB - Besides being an important oilseed crop, Brassica juncea is used as leafy vegetable, spice and biofumigation crop. To use novel breeding techniques such as genome editing, an efficient, reproducible regeneration protocol is beneficial. For B. juncea, adventitious shoot regeneration had been described previously, but mainly using lines of the Indian and Chinese gene pool. In this study, we aimed at establishing and improving an adventitious shoot regeneration protocol for current B. juncea cultivars and breeding lines from the European gene pool (‘Terraplus’, 'Terratop', ‘Terrafit’, 'Energy', SFB18/1 and SFB22/15) and compared their response to gene bank accessions originating from India. Cotyledon and hypocotyl explants of five-days-old seedlings were cultured on Murashige and Skoog medium supplemented with different concentrations BAP, IBA and NAA with or without 9.95 pM AgNO3. When the cotyledon and hypocotyl explants were cultured on media supplemented only with BAP and IBA, the regeneration rate was below 50% and 5%, respectively. The highest shoot regeneration rates of 58-72% and 65-90% for the European and Indian lines, respectively, as well as the highest shoot number of 2.7 and 3.0 per regenerating explant were obtained on a medium containing 8.88 pM BAP, 5.37 pM NAA and 9.95 pM AgNO3. Shoot regeneration rate was severely affected in all lines by ethylene production resulting from the addition of the ethylene precursor 1-aminocyclopropane-l-carboxylic acid to the medium. Also, AgNO3 caused an increased ethylene production measured after ten days of culture, but it had a significant positive effect on shoot regeneration indicating a negative effect of ethylene on early events in organogenesis.
KW - Brassicaceae
KW - brown mustard
KW - ethylene
KW - organogenesis
KW - plant growth regulators
KW - silver nitrate (AgNO)
UR - http://www.scopus.com/inward/record.url?scp=85198064105&partnerID=8YFLogxK
U2 - 10.17660/eJHS.2024/006
DO - 10.17660/eJHS.2024/006
M3 - Article
AN - SCOPUS:85198064105
VL - 89
JO - European Journal of Horticultural Science
JF - European Journal of Horticultural Science
SN - 1611-4426
IS - 2
ER -