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Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques

Research output: Contribution to journalArticleResearchpeer review

Authors

  • K. Hellmuth
  • S. Pluschkell
  • J. K. Jung
  • E. Ruttkowski
  • U. Rinas

External Research Organisations

  • Helmholtz Centre for Infection Research (HZI)
  • Technische Universität Darmstadt

Details

Original languageEnglish
Pages (from-to)978-984
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume43
Issue number6
Publication statusPublished - Nov 1995
Externally publishedYes

Abstract

Wild-type Aspergillus niger NRRL-3 was transformed with multiple copies of the glucose oxidase structural gene (god). The gene was placed under the control of the gpd A promoter of A. nidulans. For more efficient secretion the α-amylase signal peptide from A oryzae was inserted in front of god. Compared to the wild type, the recombinant strain NRRL-3 (GOD3-18) produced up to four times more extracellular glucose oxidase under identical culture conditions. Addition of yeast extract (2 g l−1) to a mineral salts medium containing only glucose as carbon source increased volumetric and specific extracellular glucose oxidase activities by 130% and 50% respectively. With the same medium composition and inoculum size, volumetric and specific extracellular glucose oxidase activities increased more than ten times in bioreactor cultivations compared to shake-flask cultures.

ASJC Scopus subject areas

Cite this

Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques. / Hellmuth, K.; Pluschkell, S.; Jung, J. K. et al.
In: Applied Microbiology and Biotechnology, Vol. 43, No. 6, 11.1995, p. 978-984.

Research output: Contribution to journalArticleResearchpeer review

Hellmuth K, Pluschkell S, Jung JK, Ruttkowski E, Rinas U. Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques. Applied Microbiology and Biotechnology. 1995 Nov;43(6):978-984. doi: 10.1007/BF00166912
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