Details
| Original language | English |
|---|---|
| Title of host publication | Chemical and Medical Sensors |
| Pages | 224-230 |
| Number of pages | 7 |
| Publication status | Published - 1 Nov 1991 |
| Event | Chemical and Medical Sensors 1991 - The Hague, Netherlands Duration: 11 Mar 1991 → 15 Mar 1991 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Publisher | SPIE |
| Volume | 1510 |
| ISSN (Print) | 0277-786X |
Abstract
The development and application of various optical sensors will be presented. Among these are optical sensors (optrodes) with immobilized enzymes, coenzymes and labeled antibodies. The NADH formation of coenzyme dependent enzymes was used for detection of lactate, pyruvate mannitol, ethanol and formate. An enzyme optrode based on a pH-optrode as a transducer for the monitoring of urea and penicillin in fermentation media was developed. For preparing an oxygen optrode, oxygen-sensitive fluorophores were entrapped in a gaspermeable silicone matrix that is attached to the distal end of a bifurcated fiber optic waveguide bundle. By labelling of immuncomponent with fluorophores or enzymes, which transpose fluorophores or chromophores, immunreactions were observed by an optical sensors.
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
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Chemical and Medical Sensors. 1991. p. 224-230 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1510).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Optical sensors for process monitoring in biotechnology
AU - Plötz, F.
AU - Schelp, C.
AU - Anders, K. D.
AU - Eberhardt, F.
AU - Scheper, T.
AU - Bückmann, F.
PY - 1991/11/1
Y1 - 1991/11/1
N2 - The development and application of various optical sensors will be presented. Among these are optical sensors (optrodes) with immobilized enzymes, coenzymes and labeled antibodies. The NADH formation of coenzyme dependent enzymes was used for detection of lactate, pyruvate mannitol, ethanol and formate. An enzyme optrode based on a pH-optrode as a transducer for the monitoring of urea and penicillin in fermentation media was developed. For preparing an oxygen optrode, oxygen-sensitive fluorophores were entrapped in a gaspermeable silicone matrix that is attached to the distal end of a bifurcated fiber optic waveguide bundle. By labelling of immuncomponent with fluorophores or enzymes, which transpose fluorophores or chromophores, immunreactions were observed by an optical sensors.
AB - The development and application of various optical sensors will be presented. Among these are optical sensors (optrodes) with immobilized enzymes, coenzymes and labeled antibodies. The NADH formation of coenzyme dependent enzymes was used for detection of lactate, pyruvate mannitol, ethanol and formate. An enzyme optrode based on a pH-optrode as a transducer for the monitoring of urea and penicillin in fermentation media was developed. For preparing an oxygen optrode, oxygen-sensitive fluorophores were entrapped in a gaspermeable silicone matrix that is attached to the distal end of a bifurcated fiber optic waveguide bundle. By labelling of immuncomponent with fluorophores or enzymes, which transpose fluorophores or chromophores, immunreactions were observed by an optical sensors.
UR - http://www.scopus.com/inward/record.url?scp=84899663106&partnerID=8YFLogxK
U2 - 10.1117/12.47142
DO - 10.1117/12.47142
M3 - Conference contribution
AN - SCOPUS:84899663106
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 224
EP - 230
BT - Chemical and Medical Sensors
T2 - Chemical and Medical Sensors 1991
Y2 - 11 March 1991 through 15 March 1991
ER -