Details
| Original language | English |
|---|---|
| Pages (from-to) | 73-82 |
| Number of pages | 10 |
| Journal | Biosensors and Bioelectronics |
| Volume | 9 |
| Issue number | 1 |
| Publication status | Published - 1994 |
| Externally published | Yes |
Abstract
Different optical sensing systems based on fluorometric detection for biotechnological purposes are presented. A fibre fluorometric sensor is described for monitoring the NADPH-dependent fluorescence of an enzyme complex from Z. mobilis for the analysis of glucose, fructose, gluconolactone or sorbitol. Optical chemosensors for pH and oxygen concentration were used as transducers for biooptodes for glucose, urea and pencillin. A two-channel device for the simultaneous monitoring of penicillin and pH is presented. Two fibre optic based immunosensors are described for the analysis of herbicides, mycotoxins and biotin. The use of the biotin sensor for the monitoring of a cultivation of recombinant E. coli is discussed.
Keywords
- bioprocess monitoring, dehydrogenases, immunosensor, multicomponent sensing, optodes
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Biosensors and Bioelectronics, Vol. 9, No. 1, 1994, p. 73-82.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Optical sensors for biotechnological applications
AU - Scheper, Th
AU - Müller, C.
AU - Anders, K. D.
AU - Eberhardt, F.
AU - Plötz, F.
AU - Schelp, C.
AU - Thordsen, O.
AU - Schügerl, K.
N1 - Funding information: The authors would like to thank Professor H. Sahm and Dr. B. Rehr for their cooperation with the fluorometric biosensor; Prof. Dr. B. Hock (TU Munchen), Prof. Dr. G. Terplan and Dr. E. Mtirtlbauer( LMU Mtinchen)f or providing the appropriate antibodies; Dr. Weissgerber, Riedel de Haen Chemicals (Hannover), for his help with the atrazine assay, and the DFG, BMFT, Ingold MesstechnikA G, and the BWB Koblenz for financial assistance.
PY - 1994
Y1 - 1994
N2 - Different optical sensing systems based on fluorometric detection for biotechnological purposes are presented. A fibre fluorometric sensor is described for monitoring the NADPH-dependent fluorescence of an enzyme complex from Z. mobilis for the analysis of glucose, fructose, gluconolactone or sorbitol. Optical chemosensors for pH and oxygen concentration were used as transducers for biooptodes for glucose, urea and pencillin. A two-channel device for the simultaneous monitoring of penicillin and pH is presented. Two fibre optic based immunosensors are described for the analysis of herbicides, mycotoxins and biotin. The use of the biotin sensor for the monitoring of a cultivation of recombinant E. coli is discussed.
AB - Different optical sensing systems based on fluorometric detection for biotechnological purposes are presented. A fibre fluorometric sensor is described for monitoring the NADPH-dependent fluorescence of an enzyme complex from Z. mobilis for the analysis of glucose, fructose, gluconolactone or sorbitol. Optical chemosensors for pH and oxygen concentration were used as transducers for biooptodes for glucose, urea and pencillin. A two-channel device for the simultaneous monitoring of penicillin and pH is presented. Two fibre optic based immunosensors are described for the analysis of herbicides, mycotoxins and biotin. The use of the biotin sensor for the monitoring of a cultivation of recombinant E. coli is discussed.
KW - bioprocess monitoring
KW - dehydrogenases
KW - immunosensor
KW - multicomponent sensing
KW - optodes
UR - http://www.scopus.com/inward/record.url?scp=0028174391&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(94)80017-0
DO - 10.1016/0956-5663(94)80017-0
M3 - Article
AN - SCOPUS:0028174391
VL - 9
SP - 73
EP - 82
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 1
ER -