Details
| Original language | English |
|---|---|
| Pages (from-to) | 133-137 |
| Number of pages | 5 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 128 |
| Issue number | 1 |
| Publication status | Published - 3 Jun 2007 |
Abstract
Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.
Keywords
- Aminofluorescein, Chemometrics, Fluorescence, pH sensing, Ratiometric sensors, Temperature dependence
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
- Materials Science(all)
- Materials Chemistry
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In: Sensors and Actuators, B: Chemical, Vol. 128, No. 1, 03.06.2007, p. 133-137.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Optical pH sensing using spectral analysis
AU - Fritzsche, M.
AU - Barreiro, C. G.
AU - Hitzmann, B.
AU - Scheper, T.
N1 - Funding information: Parts of this work were funded by the DFG. Michael Fritzsche received a PhD scholarship from the Fonds der Chemischen Industrie. The authors gratefully acknowledge this support.
PY - 2007/6/3
Y1 - 2007/6/3
N2 - Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.
AB - Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.
KW - Aminofluorescein
KW - Chemometrics
KW - Fluorescence
KW - pH sensing
KW - Ratiometric sensors
KW - Temperature dependence
UR - http://www.scopus.com/inward/record.url?scp=35748953114&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2007.05.041
DO - 10.1016/j.snb.2007.05.041
M3 - Article
AN - SCOPUS:35748953114
VL - 128
SP - 133
EP - 137
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 1
ER -