Details
| Original language | English |
|---|---|
| Title of host publication | Advances in Biochemical Engineering/Biotechnology |
| Editors | T. Scheper |
| Pages | 125-142 |
| Number of pages | 18 |
| Publication status | Published - 16 Jul 2009 |
Publication series
| Name | Advances in Biochemical Engineering/Biotechnology |
|---|---|
| Volume | 116 |
| ISSN (Print) | 0724-6145 |
Abstract
To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.
Keywords
- Cell size distribution, Focused beam reflectance measurement (FBRM), In situ microscopy, Inline monitoring, Optical sensors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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Advances in Biochemical Engineering/Biotechnology. ed. / T. Scheper. 2009. p. 125-142 (Advances in Biochemical Engineering/Biotechnology; Vol. 116).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Optical Inline Measurement Procedures for Counting and Sizing Cells in Bioprocess Technology
AU - Rudolph, Guido
AU - Lindner, Patrick
AU - Bluma, Arne
AU - Joeris, Klaus
AU - Martinez, Geovanni
AU - Hitzmann, Bernd
AU - Scheper, Thomas
PY - 2009/7/16
Y1 - 2009/7/16
N2 - To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.
AB - To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed imaging procedures is that they are only available offline which requires sampling. On the other hand, available imaging inline probes can so far only deliver a limited number of process parameters. This chapter presents new optical procedures for the inline determination of cell count, cell size distribution, and other parameters. In particular, by in situ microscopy an imaging procedure will be described which allows the determination of direct and nondirect cell parameters in real time without sampling.
KW - Cell size distribution
KW - Focused beam reflectance measurement (FBRM)
KW - In situ microscopy
KW - Inline monitoring
KW - Optical sensors
UR - http://www.scopus.com/inward/record.url?scp=77955546593&partnerID=8YFLogxK
U2 - 10.1007/10_2009_12
DO - 10.1007/10_2009_12
M3 - Contribution to book/anthology
C2 - 19609497
AN - SCOPUS:77955546593
SN - 9783642008641
T3 - Advances in Biochemical Engineering/Biotechnology
SP - 125
EP - 142
BT - Advances in Biochemical Engineering/Biotechnology
A2 - Scheper, T.
ER -