Details
| Original language | English |
|---|---|
| Pages (from-to) | 136-145 |
| Number of pages | 10 |
| Journal | Biotechnology and Bioengineering |
| Volume | 99 |
| Issue number | 1 |
| Publication status | Published - 1 Jun 2007 |
Abstract
Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations. In this work the in situ microscope was utilized to measure the level of colonization of fibroblasts on microcarrier surfaces during cultivation. For this study the murine cell line NIH-3T3 was used in combination with Cytodex 1 microcarriers. Cultivations were carried out in a 5 L stirred tank bioreactor equipped with the in situ microscope. Images were obtained sequentially with the in situ microscope over the whole cultivation time (900 images per sequence, 7.5 h per sequence on average). For the microcarrier analysis an image analysis algorithm based on a neural network was developed and implemented in the microscope analysis software.
Keywords
- Fibroblasts, Image analysis, In situ microscopy, Inline estimation, Level of colonization, Microcarriers, Neural network
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Biotechnology and Bioengineering, Vol. 99, No. 1, 01.06.2007, p. 136-145.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Online Monitoring of Microcarrier Based Fibroblast Cultivations With In Situ Microscopy
AU - Rudolph, Guido
AU - Lindner, Patrick
AU - Gierse, Alexander
AU - Bluma, Arne
AU - Martinez, Geovanni
AU - Hitzmann, Bernd
AU - Scheper, Thomas
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations. In this work the in situ microscope was utilized to measure the level of colonization of fibroblasts on microcarrier surfaces during cultivation. For this study the murine cell line NIH-3T3 was used in combination with Cytodex 1 microcarriers. Cultivations were carried out in a 5 L stirred tank bioreactor equipped with the in situ microscope. Images were obtained sequentially with the in situ microscope over the whole cultivation time (900 images per sequence, 7.5 h per sequence on average). For the microcarrier analysis an image analysis algorithm based on a neural network was developed and implemented in the microscope analysis software.
AB - Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations. In this work the in situ microscope was utilized to measure the level of colonization of fibroblasts on microcarrier surfaces during cultivation. For this study the murine cell line NIH-3T3 was used in combination with Cytodex 1 microcarriers. Cultivations were carried out in a 5 L stirred tank bioreactor equipped with the in situ microscope. Images were obtained sequentially with the in situ microscope over the whole cultivation time (900 images per sequence, 7.5 h per sequence on average). For the microcarrier analysis an image analysis algorithm based on a neural network was developed and implemented in the microscope analysis software.
KW - Fibroblasts
KW - Image analysis
KW - In situ microscopy
KW - Inline estimation
KW - Level of colonization
KW - Microcarriers
KW - Neural network
UR - http://www.scopus.com/inward/record.url?scp=37549064018&partnerID=8YFLogxK
U2 - 10.1002/bit.21523
DO - 10.1002/bit.21523
M3 - Article
C2 - 17546690
AN - SCOPUS:37549064018
VL - 99
SP - 136
EP - 145
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 1
ER -