Details
| Original language | English |
|---|---|
| Pages (from-to) | 83-85 |
| Number of pages | 3 |
| Journal | Journal of biotechnology |
| Volume | 259 |
| Publication status | Published - 2 Aug 2017 |
Abstract
In situ Microscopy (ISM) is an optical non-invasive technique to monitor cells in bioprocesses in real-time. Escherichia coli is the most studied and used organism in biotechnology. In this article the cell density in Escherichia coli cultivations was monitored by applying ISM in these cultivations. The acquired images were analyzed with an image processing algorithm to determine the turbidity of the cultivation medium. In three cultivations the cell density was monitored with the algorithm and offline samples were taken to determine the dry cell mass (DCM). Both results were correlated and concentrations up to 70 g/L DCM could be measured via ISM. For higher cell densities a saturation was recognized. The deviation of the calibration lines within three cultivations was 8%.
Keywords
- Escherichia coli, Image processing, In situ microscopy, Online monitoring
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 259, 02.08.2017, p. 83-85.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Online monitoring of cell concentration in high cell density Escherichia coli cultivations using in situ Microscopy
AU - Marquard, D.
AU - Schneider-Barthold, C.
AU - Düsterloh, S.
AU - Scheper, T.
AU - Lindner, P.
N1 - Funding information: This work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH.
PY - 2017/8/2
Y1 - 2017/8/2
N2 - In situ Microscopy (ISM) is an optical non-invasive technique to monitor cells in bioprocesses in real-time. Escherichia coli is the most studied and used organism in biotechnology. In this article the cell density in Escherichia coli cultivations was monitored by applying ISM in these cultivations. The acquired images were analyzed with an image processing algorithm to determine the turbidity of the cultivation medium. In three cultivations the cell density was monitored with the algorithm and offline samples were taken to determine the dry cell mass (DCM). Both results were correlated and concentrations up to 70 g/L DCM could be measured via ISM. For higher cell densities a saturation was recognized. The deviation of the calibration lines within three cultivations was 8%.
AB - In situ Microscopy (ISM) is an optical non-invasive technique to monitor cells in bioprocesses in real-time. Escherichia coli is the most studied and used organism in biotechnology. In this article the cell density in Escherichia coli cultivations was monitored by applying ISM in these cultivations. The acquired images were analyzed with an image processing algorithm to determine the turbidity of the cultivation medium. In three cultivations the cell density was monitored with the algorithm and offline samples were taken to determine the dry cell mass (DCM). Both results were correlated and concentrations up to 70 g/L DCM could be measured via ISM. For higher cell densities a saturation was recognized. The deviation of the calibration lines within three cultivations was 8%.
KW - Escherichia coli
KW - Image processing
KW - In situ microscopy
KW - Online monitoring
UR - http://www.scopus.com/inward/record.url?scp=85028316186&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2017.08.003
DO - 10.1016/j.jbiotec.2017.08.003
M3 - Article
C2 - 28780163
AN - SCOPUS:85028316186
VL - 259
SP - 83
EP - 85
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
ER -