Details
| Original language | English |
|---|---|
| Pages (from-to) | 395-403 |
| Number of pages | 9 |
| Journal | Journal of biotechnology |
| Volume | 31 |
| Issue number | 3 |
| Publication status | Published - Dec 1993 |
| Externally published | Yes |
Abstract
Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.
Keywords
- FIA, Flow injection analysis, On-line monitoring, Process control, Tissue-type plasminogen activator, Turbidimetric immunoassay
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 31, No. 3, 12.1993, p. 395-403.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - On-line immunoanalysis for bioprocess control
AU - Middendorf, C.
AU - Schulze, B.
AU - Freitag, R.
AU - Scheper, Th
AU - Howaldt, M.
AU - Hoffmann, H.
PY - 1993/12
Y1 - 1993/12
N2 - Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.
AB - Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.
KW - FIA
KW - Flow injection analysis
KW - On-line monitoring
KW - Process control
KW - Tissue-type plasminogen activator
KW - Turbidimetric immunoassay
UR - http://www.scopus.com/inward/record.url?scp=0027743394&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(93)90083-Y
DO - 10.1016/0168-1656(93)90083-Y
M3 - Article
C2 - 7764446
AN - SCOPUS:0027743394
VL - 31
SP - 395
EP - 403
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 3
ER -