Details
| Original language | English |
|---|---|
| Pages (from-to) | 139-143 |
| Number of pages | 5 |
| Journal | Journal of biotechnology |
| Volume | 46 |
| Issue number | 2 |
| Publication status | Published - 30 Apr 1996 |
Abstract
Lipase-catalyzed kinetic resolution of 3-hydroxyoctanoic acid methylester in supercritical carbon dioxide has been investigated. The reaction progress was monitored on-line following the absorbance of the by-product acetic aldehyde using a UV photometer equipped with a high pressure flow through cell. The conversion determined by this method was in very good accordance with results obtained by off-line sampling and subsequent analysis by gas chromatography using a chiral column.
Keywords
- 3-Hydroxy ester, Kinetic resolution, Lipase, On-line analysis, Supercritical carbon dioxide
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 46, No. 2, 30.04.1996, p. 139-143.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - On-line determination of the conversion in a lipase-catalyzed kinetic resolution in supercritical carbon dioxide
AU - Bornscheuer, U.
AU - Capewell, A.
AU - Wendel, V.
AU - Scheper, T.
PY - 1996/4/30
Y1 - 1996/4/30
N2 - Lipase-catalyzed kinetic resolution of 3-hydroxyoctanoic acid methylester in supercritical carbon dioxide has been investigated. The reaction progress was monitored on-line following the absorbance of the by-product acetic aldehyde using a UV photometer equipped with a high pressure flow through cell. The conversion determined by this method was in very good accordance with results obtained by off-line sampling and subsequent analysis by gas chromatography using a chiral column.
AB - Lipase-catalyzed kinetic resolution of 3-hydroxyoctanoic acid methylester in supercritical carbon dioxide has been investigated. The reaction progress was monitored on-line following the absorbance of the by-product acetic aldehyde using a UV photometer equipped with a high pressure flow through cell. The conversion determined by this method was in very good accordance with results obtained by off-line sampling and subsequent analysis by gas chromatography using a chiral column.
KW - 3-Hydroxy ester
KW - Kinetic resolution
KW - Lipase
KW - On-line analysis
KW - Supercritical carbon dioxide
UR - http://www.scopus.com/inward/record.url?scp=0030131030&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(95)00195-6
DO - 10.1016/0168-1656(95)00195-6
M3 - Article
AN - SCOPUS:0030131030
VL - 46
SP - 139
EP - 143
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2
ER -