Details
| Original language | English |
|---|---|
| Pages (from-to) | 15129-15136 |
| Number of pages | 8 |
| Journal | Analytical chemistry |
| Volume | 92 |
| Issue number | 22 |
| Early online date | 4 Nov 2020 |
| Publication status | Published - 17 Nov 2020 |
Abstract
We report the first hyphenation of chip-electrochromatography (ChEC) with ion mobility spectrometry (IMS). This approach combines the separation power of two electrokinetically driven separation techniques, the first in liquid phase and the second in gas phase, with a label-free detection of the analytes. For achieving this, a microfluidic glass chip incorporating a monolithic separation column, a nanofluidic liquid junction for providing post-column electrical contact, and a monolithically integrated electrospray emitter was developed. This device was successfully coupled to a custom-built high-resolution drift tube IMS with shifted potentials. After proof-of-concept studies in which a mixture of five model compounds was analyzed in less than 80 s, this first ChEC−IMS system was applied to a more complex sample, the analysis of herbicides spiked in the wine matrix. The use of ChEC before IMS detection not only facilitated the peak allocation and increased the peak capacity but also enabled analyte quantification. As both, ChEC and IMS work at ambient conditions and are driven by high voltages, no bulky pumping systems are needed, neither for the hydrodynamic pumping of the mobile phase as in high-performance liquid chromatography nor for generating a vacuum system as in mass spectrometry. Accordingly, the approach has great potential as a portable analytical system for field analysis of complex mixtures.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
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In: Analytical chemistry, Vol. 92, No. 22, 17.11.2020, p. 15129-15136.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - On-Line Coupling of Chip-Electrochromatography and Ion Mobility Spectrometry
AU - Hartner, Nora T.
AU - Raddatz, Christian Robert
AU - Thoben, Christian
AU - Piendl, Sebastian K.
AU - Zimmermann, Stefan
AU - Belder, Detlev
N1 - Funding Information: This work was funded by the Deutsche Forschungsgemeinschaft (DFG) Project numbers 396430937, 371350234, and FOR 2177. We would also like to thank Alexander Bohnhorst and Ansgar T. Kirk for their contributions regarding data evaluation.
PY - 2020/11/17
Y1 - 2020/11/17
N2 - We report the first hyphenation of chip-electrochromatography (ChEC) with ion mobility spectrometry (IMS). This approach combines the separation power of two electrokinetically driven separation techniques, the first in liquid phase and the second in gas phase, with a label-free detection of the analytes. For achieving this, a microfluidic glass chip incorporating a monolithic separation column, a nanofluidic liquid junction for providing post-column electrical contact, and a monolithically integrated electrospray emitter was developed. This device was successfully coupled to a custom-built high-resolution drift tube IMS with shifted potentials. After proof-of-concept studies in which a mixture of five model compounds was analyzed in less than 80 s, this first ChEC−IMS system was applied to a more complex sample, the analysis of herbicides spiked in the wine matrix. The use of ChEC before IMS detection not only facilitated the peak allocation and increased the peak capacity but also enabled analyte quantification. As both, ChEC and IMS work at ambient conditions and are driven by high voltages, no bulky pumping systems are needed, neither for the hydrodynamic pumping of the mobile phase as in high-performance liquid chromatography nor for generating a vacuum system as in mass spectrometry. Accordingly, the approach has great potential as a portable analytical system for field analysis of complex mixtures.
AB - We report the first hyphenation of chip-electrochromatography (ChEC) with ion mobility spectrometry (IMS). This approach combines the separation power of two electrokinetically driven separation techniques, the first in liquid phase and the second in gas phase, with a label-free detection of the analytes. For achieving this, a microfluidic glass chip incorporating a monolithic separation column, a nanofluidic liquid junction for providing post-column electrical contact, and a monolithically integrated electrospray emitter was developed. This device was successfully coupled to a custom-built high-resolution drift tube IMS with shifted potentials. After proof-of-concept studies in which a mixture of five model compounds was analyzed in less than 80 s, this first ChEC−IMS system was applied to a more complex sample, the analysis of herbicides spiked in the wine matrix. The use of ChEC before IMS detection not only facilitated the peak allocation and increased the peak capacity but also enabled analyte quantification. As both, ChEC and IMS work at ambient conditions and are driven by high voltages, no bulky pumping systems are needed, neither for the hydrodynamic pumping of the mobile phase as in high-performance liquid chromatography nor for generating a vacuum system as in mass spectrometry. Accordingly, the approach has great potential as a portable analytical system for field analysis of complex mixtures.
UR - http://www.scopus.com/inward/record.url?scp=85098926552&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c03446
DO - 10.1021/acs.analchem.0c03446
M3 - Article
AN - SCOPUS:85098926552
VL - 92
SP - 15129
EP - 15136
JO - Analytical chemistry
JF - Analytical chemistry
SN - 0003-2700
IS - 22
ER -