Details
| Original language | English |
|---|---|
| Article number | 120111 |
| Journal | TALANTA |
| Volume | 205 |
| Early online date | 2 Jul 2019 |
| Publication status | Published - 1 Dec 2019 |
| Externally published | Yes |
Abstract
Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 109 (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.
Keywords
- Adult, Aged, Biosensing Techniques/methods, Cadmium Compounds/chemistry, Cell Line, Tumor, DNA Probes/chemistry, DNA, Viral/blood, Glass/chemistry, Humans, Limit of Detection, Magnetic Phenomena, Male, Microscopy, Fluorescence/methods, Nucleic Acid Hybridization, Papillomaviridae/chemistry, Papillomavirus Infections/diagnosis, Quantum Dots/chemistry, Spectrometry, Fluorescence/methods, Squamous Cell Carcinoma of Head and Neck/virology, Tellurium/chemistry, Human papillomavirus, Magnetic isolation, Quantum dots, Nanotechnology, Cancer
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
Sustainable Development Goals
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In: TALANTA, Vol. 205, 120111, 01.12.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity
AU - Jimenez Jimenez, Ana Maria
AU - Moulick, Amitava
AU - Bhowmick, Sukanya
AU - Strmiska, Vladislav
AU - Gagic, Milica
AU - Horakova, Zuzana
AU - Kostrica, Rom
AU - Masarik, Michal
AU - Heger, Zbynek
AU - Adam, Vojtech
N1 - Funding information: This research was carried out under the project CEITEC 2020 ( LQ1601 ) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II, by the Ministry of Health of the Czech Republic ( 16-29835A ) and by the Grant Agency of the Czech Republic ( 18-03978S ). This research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II, by the Ministry of Health of the Czech Republic (16-29835A) and by the Grant Agency of the Czech Republic (18-03978S).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 109 (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.
AB - Due to the close relationship between carcinogenesis and human papillomavirus (HPV), and since they are transmitted via huge number of asymptomatic carriers, the detection of HPV is really needed to reduce the risk of developing cancer. According to the best of our knowledge, our study provides the very first method for one-step detection of viral infection and if it has initiated the subsequent cancer proliferation. The proposed novel nanosystem consists of magnetic glass particles (MGPs), which were attached with DNA probe on their surface to hybridize with target DNAs. The MGP-probe-DNA hybrid was finally conjugated with CdTe/ZnSe core/shell quantum dots (QDs). The proposed detection system is based on a novel mechanism in which the MGPs separate out the target DNAs from different biological samples using external magnetic field for better and clear detection and the QDs give different fluorescent maxima for different target DNAs due to their ability to interact differently with different nucleotides. Firstly, the method was optimized using HPV genes cloned into synthetic plasmids. Then it was applied directly on the samples from normal and cancerous cells. After that, the real hospital samples of head and neck squamous cell carcinoma (HNSCC) with or without the infection of HPV were also analyzed. Our novel nano-system is proved successful in detecting and distinguishing between the patients suffering by HPV infection with or without subsequent cancer having detection limit estimated as 1.0 x 109 (GEq/mL). The proposed methodology is faster and cost-effective, which can be applied at the clinical level to help the doctors to decide the strategy of medication that may save the life of the patients with an early treatment.
KW - Adult
KW - Aged
KW - Biosensing Techniques/methods
KW - Cadmium Compounds/chemistry
KW - Cell Line, Tumor
KW - DNA Probes/chemistry
KW - DNA, Viral/blood
KW - Glass/chemistry
KW - Humans
KW - Limit of Detection
KW - Magnetic Phenomena
KW - Male
KW - Microscopy, Fluorescence/methods
KW - Nucleic Acid Hybridization
KW - Papillomaviridae/chemistry
KW - Papillomavirus Infections/diagnosis
KW - Quantum Dots/chemistry
KW - Spectrometry, Fluorescence/methods
KW - Squamous Cell Carcinoma of Head and Neck/virology
KW - Tellurium/chemistry
KW - Human papillomavirus
KW - Magnetic isolation
KW - Quantum dots
KW - Nanotechnology
KW - Cancer
UR - http://www.scopus.com/inward/record.url?scp=85068523227&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2019.07.006
DO - 10.1016/j.talanta.2019.07.006
M3 - Article
C2 - 31450441
VL - 205
JO - TALANTA
JF - TALANTA
SN - 0039-9140
M1 - 120111
ER -