Details
| Original language | English |
|---|---|
| Pages (from-to) | 1767-1773 |
| Number of pages | 7 |
| Journal | ACS SENSORS |
| Volume | 2 |
| Issue number | 12 |
| Publication status | Published - 22 Nov 2017 |
Abstract
Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors' performance, specifically in terms of their sensitivity (which is typically in the micromolar range), is insufficient for many applications. Herein, we present a proof-of-concept application of the electrokinetic isotachophoresis (ITP) technique for real-time preconcentration of a target protein on a PSi biosensor. With ITP, a highly concentrated target zone is delivered to the sensing area, where the protein target is captured by immobilized aptamers. The detection of the binding events is conducted in a label-free manner by reflective interferometric Fourier transformation spectroscopy (RIFTS). Up to 1000-fold enhancement in local concentration of the protein target and the biosensor's sensitivity are achieved, with a measured limit of detection of 7.5 nM. Furthermore, the assay is successfully performed in complex media, such as bacteria lysate samples, while the selectivity of the biosensor is retained. The presented assay could be further utilized for other protein targets, and to promote the development of clinically useful PSi biosensors.
Keywords
- aptamer, isotachophoresis, label-free, optical biosensor, porous silicon
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Physics and Astronomy(all)
- Instrumentation
- Chemical Engineering(all)
- Process Chemistry and Technology
- Chemical Engineering(all)
- Fluid Flow and Transfer Processes
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In: ACS SENSORS, Vol. 2, No. 12, 22.11.2017, p. 1767-1773.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - On Chip Protein Pre-Concentration for Enhancing the Sensitivity of Porous Silicon Biosensors
AU - Arshavsky-Graham, Sofia
AU - Massad-Ivanir, Naama
AU - Paratore, Federico
AU - Scheper, Thomas
AU - Bercovici, Moran
AU - Segal, Ester
N1 - Funding information: This work was partially supported by the NEVET grant administrated by the Russell Berrie Nanotechnology Institute (RBNI), by the German Research Foundation under the grant SCHE 279/32-1 and by the Initial Training Network, Virtual Vials, funded by the FP7 Marie Curie Actions of the European Commission (FP7-PEOPLE-2013-ITN-607322). The oxidation process was performed at the Micro-Nano Fabrication Unit (MNFU), Technion. The authors thank Dr. Khaled Gommed for his assistance in preparation of the microfluidic channels and Prof. Yuval Shoham from the Department of Biotechnology and Food Engineering at the Technion for supplying the target protein. S.A.-G. is most grateful for the Miriam and Aaron Gutwirth scholarship and for TEVA Pharmaceutical Industries Excellence Scholarship for M.Sc. students in Analytical Chemistry.
PY - 2017/11/22
Y1 - 2017/11/22
N2 - Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors' performance, specifically in terms of their sensitivity (which is typically in the micromolar range), is insufficient for many applications. Herein, we present a proof-of-concept application of the electrokinetic isotachophoresis (ITP) technique for real-time preconcentration of a target protein on a PSi biosensor. With ITP, a highly concentrated target zone is delivered to the sensing area, where the protein target is captured by immobilized aptamers. The detection of the binding events is conducted in a label-free manner by reflective interferometric Fourier transformation spectroscopy (RIFTS). Up to 1000-fold enhancement in local concentration of the protein target and the biosensor's sensitivity are achieved, with a measured limit of detection of 7.5 nM. Furthermore, the assay is successfully performed in complex media, such as bacteria lysate samples, while the selectivity of the biosensor is retained. The presented assay could be further utilized for other protein targets, and to promote the development of clinically useful PSi biosensors.
AB - Porous silicon (PSi) nanomaterials have been widely studied as label-free optical biosensors for protein detection. However, these biosensors' performance, specifically in terms of their sensitivity (which is typically in the micromolar range), is insufficient for many applications. Herein, we present a proof-of-concept application of the electrokinetic isotachophoresis (ITP) technique for real-time preconcentration of a target protein on a PSi biosensor. With ITP, a highly concentrated target zone is delivered to the sensing area, where the protein target is captured by immobilized aptamers. The detection of the binding events is conducted in a label-free manner by reflective interferometric Fourier transformation spectroscopy (RIFTS). Up to 1000-fold enhancement in local concentration of the protein target and the biosensor's sensitivity are achieved, with a measured limit of detection of 7.5 nM. Furthermore, the assay is successfully performed in complex media, such as bacteria lysate samples, while the selectivity of the biosensor is retained. The presented assay could be further utilized for other protein targets, and to promote the development of clinically useful PSi biosensors.
KW - aptamer
KW - isotachophoresis
KW - label-free
KW - optical biosensor
KW - porous silicon
UR - http://www.scopus.com/inward/record.url?scp=85039169604&partnerID=8YFLogxK
U2 - 10.1021/acssensors.7b00692
DO - 10.1021/acssensors.7b00692
M3 - Article
C2 - 29164872
AN - SCOPUS:85039169604
VL - 2
SP - 1767
EP - 1773
JO - ACS SENSORS
JF - ACS SENSORS
SN - 2379-3694
IS - 12
ER -