Details
| Original language | English |
|---|---|
| Pages (from-to) | 187-188 |
| Number of pages | 2 |
| Journal | VIRUS GENES |
| Volume | 38 |
| Issue number | 1 |
| Publication status | Published - 20 Nov 2008 |
Abstract
Carrot motley dwarf (CMD) is known to result from a mixed infection by two viruses, the polerovirus Carrot red leaf virus and one of the umbraviruses Carrot mottle mimic virus or Carrot mottle virus. Some umbraviruses have been shown to be associated with small satellite (sat) RNAs, but none have been reported for the latter two. A CMD-affected parsley plant was used for sap transmission to test plants, that were used for dsRNA isolation. The presence of a 0.8-kbp dsRNA indicated the occurrence of a hitherto unrecognized satRNA associated with CMD. The satRNAs of the CMD isolate from parsley and an isolate from carrot have been sequenced and showed 94% sequence identity. Nucleotide sequences and putative translation products had no significant similarities to GenBank entries. To our knowledge, this is the first report of satRNAs associated with CMD.
Keywords
- Carrot motley dwarf, Carrot mottle mimic virus, Carrot mottle virus, Satellite RNA
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Immunology and Microbiology(all)
- Virology
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In: VIRUS GENES, Vol. 38, No. 1, 20.11.2008, p. 187-188.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Nucleotide sequence of a satellite RNA associated with carrot motley dwarf in parsley and carrot
AU - Menzel, Wulf
AU - Maiss, Edgar
AU - Vetten, H. Josef
PY - 2008/11/20
Y1 - 2008/11/20
N2 - Carrot motley dwarf (CMD) is known to result from a mixed infection by two viruses, the polerovirus Carrot red leaf virus and one of the umbraviruses Carrot mottle mimic virus or Carrot mottle virus. Some umbraviruses have been shown to be associated with small satellite (sat) RNAs, but none have been reported for the latter two. A CMD-affected parsley plant was used for sap transmission to test plants, that were used for dsRNA isolation. The presence of a 0.8-kbp dsRNA indicated the occurrence of a hitherto unrecognized satRNA associated with CMD. The satRNAs of the CMD isolate from parsley and an isolate from carrot have been sequenced and showed 94% sequence identity. Nucleotide sequences and putative translation products had no significant similarities to GenBank entries. To our knowledge, this is the first report of satRNAs associated with CMD.
AB - Carrot motley dwarf (CMD) is known to result from a mixed infection by two viruses, the polerovirus Carrot red leaf virus and one of the umbraviruses Carrot mottle mimic virus or Carrot mottle virus. Some umbraviruses have been shown to be associated with small satellite (sat) RNAs, but none have been reported for the latter two. A CMD-affected parsley plant was used for sap transmission to test plants, that were used for dsRNA isolation. The presence of a 0.8-kbp dsRNA indicated the occurrence of a hitherto unrecognized satRNA associated with CMD. The satRNAs of the CMD isolate from parsley and an isolate from carrot have been sequenced and showed 94% sequence identity. Nucleotide sequences and putative translation products had no significant similarities to GenBank entries. To our knowledge, this is the first report of satRNAs associated with CMD.
KW - Carrot motley dwarf
KW - Carrot mottle mimic virus
KW - Carrot mottle virus
KW - Satellite RNA
UR - http://www.scopus.com/inward/record.url?scp=58149458641&partnerID=8YFLogxK
U2 - 10.1007/s11262-008-0302-5
DO - 10.1007/s11262-008-0302-5
M3 - Article
C2 - 19020967
AN - SCOPUS:58149458641
VL - 38
SP - 187
EP - 188
JO - VIRUS GENES
JF - VIRUS GENES
SN - 0920-8569
IS - 1
ER -