Details
| Original language | English |
|---|---|
| Article number | e104941 |
| Journal | The EMBO journal |
| Volume | 39 |
| Issue number | 22 |
| Publication status | Published - 16 Nov 2020 |
Abstract
The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi-autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid-encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome-mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre-sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B-mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP-driven chloroplastic gene expression during chloroplast biogenesis.
Keywords
- Acid Phosphatase/genetics, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Cell Nucleus/metabolism, Chloroplasts/genetics, DNA-Directed RNA Polymerases/metabolism, Gene Expression Regulation, Plant, Light, Morphogenesis/physiology, Organelle Biogenesis, Phytochrome/metabolism, Plants, Genetically Modified, Plastids/genetics, Signal Transduction, Transcription Factors, Transcription, Genetic, photobodies, Arabidopsis PEP/PAPs, biogenesis, photomorphogenesis, chloroplast
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Neuroscience(all)
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In: The EMBO journal, Vol. 39, No. 22, e104941, 16.11.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Nucleo-plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis
AU - Liebers, Monique
AU - Gillet, François-Xavier
AU - Israel, Abir
AU - Pounot, Kevin
AU - Chambon, Louise
AU - Chieb, Maha
AU - Chevalier, Fabien
AU - Ruedas, Rémi
AU - Favier, Adrien
AU - Gans, Pierre
AU - Boeri Erba, Elisabetta
AU - Cobessi, David
AU - Pfannschmidt, Thomas
AU - Blanvillain, Robert
N1 - Funding Information: We acknowledge the platforms of the Grenoble Instruct‐ERIC Centre (ISBG; UMS 3518 CNRS‐CEA‐UGA‐EMBL) within the Grenoble Partnership for Structural Biology (PSB). Platform access was supported by FRISBI (ANR‐10‐INBS‐05‐02) and GRAL, a project of the University Grenoble Alpes Graduate School (Ecoles Universitaires de Recherche) CBH‐EUR‐GS (ANR‐17‐EURE‐0003). IBS acknowledges integration into the Interdisciplinary Research Institute of Grenoble (IRIG, CEA). The work was supported by the (grant PepRegulChloro3D), the to T.P. (PF323‐5) and the AGIR programme of (UGA) to R.B. The project received further support by institutional grants to the by Labex Grenoble Alliance of Integrated Structural Biology (GRAL) and ANR‐17‐EURE‐0003. We thank F Barneche for the ChIP‐seq analysis at the PAP8 locus; E Monte and G Toledo‐Ortiz for seeds; A Guerrero‐Criado, R Toutain and S Coveley for their help at the bench. We thank E Thevenon in the Parcy Lab for advice on fluorescent labelling EMSA. We thank S Lerbs‐Mache for critical reading. We express our gratitude in memory of D Grunwald for his help on confocal imaging. Agence National de la Recherche Deutsche Forschungsgemeinschaft Université Grenoble‐Alpes Laboratoire de Physiologie Cellulaire et Végétale pifq
PY - 2020/11/16
Y1 - 2020/11/16
N2 - The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi-autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid-encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome-mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre-sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B-mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP-driven chloroplastic gene expression during chloroplast biogenesis.
AB - The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi-autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid-encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome-mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre-sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B-mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP-driven chloroplastic gene expression during chloroplast biogenesis.
KW - Acid Phosphatase/genetics
KW - Arabidopsis/genetics
KW - Arabidopsis Proteins/genetics
KW - Cell Nucleus/metabolism
KW - Chloroplasts/genetics
KW - DNA-Directed RNA Polymerases/metabolism
KW - Gene Expression Regulation, Plant
KW - Light
KW - Morphogenesis/physiology
KW - Organelle Biogenesis
KW - Phytochrome/metabolism
KW - Plants, Genetically Modified
KW - Plastids/genetics
KW - Signal Transduction
KW - Transcription Factors
KW - Transcription, Genetic
KW - photobodies
KW - Arabidopsis PEP/PAPs
KW - biogenesis
KW - photomorphogenesis
KW - chloroplast
UR - http://www.scopus.com/inward/record.url?scp=85091725380&partnerID=8YFLogxK
U2 - 10.15252/embj.2020104941
DO - 10.15252/embj.2020104941
M3 - Article
C2 - 33001465
VL - 39
JO - The EMBO journal
JF - The EMBO journal
SN - 0261-4189
IS - 22
M1 - e104941
ER -