Details
| Original language | English |
|---|---|
| Title of host publication | XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010) |
| Subtitle of host publication | International Symposium on Genomics and Genetic Transformation of Horticultural Crops |
| Publisher | International Society for Horticultural Science |
| Pages | 157-162 |
| Number of pages | 6 |
| ISBN (print) | 9789066050488 |
| Publication status | Published - 31 Mar 2012 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 929 |
| ISSN (Print) | 0567-7572 |
Abstract
The genus Helleborus comprises 22 species which are allocated to six sections. Due to high intraspecific morphological variability, taxonomic and genetic relationships are ambiguous especially within section Helleborastrum. In order to investigate taxonomic and genetic relation-ships in the genus Helleborus, flow cytometry and amplified fragment length polymorphisms were used. Nuclear DNA contents of 21 analyzed species varied between 18.3 pg/2C for H. argutifolius and 32.7 pg/2C for H. thibetanus. Genome sizes between 26.8 and 31.3 pg/2C were determined for the majority of species. The genetic relationships within the genus were analyzed using ten AFLP primer combinations for 19 out of 22 Helleborus species. On the basis of 1109 polymorphic fragments across all species and the outgroup Pulsatilla vulgaris 'Violet', genetic distances were determined to conduct neighbor-joining cluster and bootstrap analysis to calculate a majority rule consensus tree. The six Helleborus sections were clearly definable in the phenogram that thereby supports the present genus division.
Keywords
- Flow cytometry, Genome size, Helleborus, Molecular marker, Taxonomy
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops. International Society for Horticultural Science, 2012. p. 157-162 (Acta Horticulturae; Vol. 929).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Nuclear DNA content and genetic relationships based on AFLP data in Helleborus
AU - Meiners, J.
AU - Debener, T.
AU - Schweizer, G.
AU - Winkelmann, T.
PY - 2012/3/31
Y1 - 2012/3/31
N2 - The genus Helleborus comprises 22 species which are allocated to six sections. Due to high intraspecific morphological variability, taxonomic and genetic relationships are ambiguous especially within section Helleborastrum. In order to investigate taxonomic and genetic relation-ships in the genus Helleborus, flow cytometry and amplified fragment length polymorphisms were used. Nuclear DNA contents of 21 analyzed species varied between 18.3 pg/2C for H. argutifolius and 32.7 pg/2C for H. thibetanus. Genome sizes between 26.8 and 31.3 pg/2C were determined for the majority of species. The genetic relationships within the genus were analyzed using ten AFLP primer combinations for 19 out of 22 Helleborus species. On the basis of 1109 polymorphic fragments across all species and the outgroup Pulsatilla vulgaris 'Violet', genetic distances were determined to conduct neighbor-joining cluster and bootstrap analysis to calculate a majority rule consensus tree. The six Helleborus sections were clearly definable in the phenogram that thereby supports the present genus division.
AB - The genus Helleborus comprises 22 species which are allocated to six sections. Due to high intraspecific morphological variability, taxonomic and genetic relationships are ambiguous especially within section Helleborastrum. In order to investigate taxonomic and genetic relation-ships in the genus Helleborus, flow cytometry and amplified fragment length polymorphisms were used. Nuclear DNA contents of 21 analyzed species varied between 18.3 pg/2C for H. argutifolius and 32.7 pg/2C for H. thibetanus. Genome sizes between 26.8 and 31.3 pg/2C were determined for the majority of species. The genetic relationships within the genus were analyzed using ten AFLP primer combinations for 19 out of 22 Helleborus species. On the basis of 1109 polymorphic fragments across all species and the outgroup Pulsatilla vulgaris 'Violet', genetic distances were determined to conduct neighbor-joining cluster and bootstrap analysis to calculate a majority rule consensus tree. The six Helleborus sections were clearly definable in the phenogram that thereby supports the present genus division.
KW - Flow cytometry
KW - Genome size
KW - Helleborus
KW - Molecular marker
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=84863620848&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2012.929.22
DO - 10.17660/ActaHortic.2012.929.22
M3 - Conference contribution
AN - SCOPUS:84863620848
SN - 9789066050488
T3 - Acta Horticulturae
SP - 157
EP - 162
BT - XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010)
PB - International Society for Horticultural Science
ER -