Details
| Original language | German |
|---|---|
| Pages (from-to) | 325-329 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 2003 |
| Issue number | 793:2 |
| Publication status | Published - 2003 |
Abstract
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 2003, No. 793:2, 2003, p. 325-329.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Novel purification system for 6xHis-tagged proteins by magnetic affinity separation
AU - Reinard, Thomas
AU - Frenzel, André
AU - Köhl, Gabriele
AU - Bergemann, Christian
PY - 2003
Y1 - 2003
N2 - We have developed a novel nickel-silica matrix for the generation of magnetic beads for metal-ion affinity chromatography. In contrast to magnetic Ni-NTA agarose beads, the novel particle type (SiMAC) consists of a magnetic core and a nickel-silica composite matrix with the nickel ions tightly integrated in the silica. This results in a much higher number of chelating groups compared with Ni-NTA agarose beads. With the SiMAC beads, greatly improved purification of histidine-tagged proteins from crude bacterial extracts was achieved. The yield was at least twice as high as with conventional materials, the method is faster, since the coupling step is omitted and there is no need for handling toxic Ni2+ salts. © 2003 Elsevier B.V. All rights reserved.
AB - We have developed a novel nickel-silica matrix for the generation of magnetic beads for metal-ion affinity chromatography. In contrast to magnetic Ni-NTA agarose beads, the novel particle type (SiMAC) consists of a magnetic core and a nickel-silica composite matrix with the nickel ions tightly integrated in the silica. This results in a much higher number of chelating groups compared with Ni-NTA agarose beads. With the SiMAC beads, greatly improved purification of histidine-tagged proteins from crude bacterial extracts was achieved. The yield was at least twice as high as with conventional materials, the method is faster, since the coupling step is omitted and there is no need for handling toxic Ni2+ salts. © 2003 Elsevier B.V. All rights reserved.
U2 - 10.1016/S1570-0232(03)00332-5
DO - 10.1016/S1570-0232(03)00332-5
M3 - Artikel
VL - 2003
SP - 325
EP - 329
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 793:2
ER -