Details
| Original language | English |
|---|---|
| Pages (from-to) | 267-273 |
| Number of pages | 7 |
| Journal | Materials Science and Engineering C |
| Volume | 41 |
| Publication status | Published - 1 Aug 2014 |
Abstract
Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to < 70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.
Keywords
- Cytotoxicity, Degradation, In vitro, Magnesium alloy, Orthopedic
ASJC Scopus subject areas
- Materials Science(all)
- General Materials Science
- Physics and Astronomy(all)
- Condensed Matter Physics
- Engineering(all)
- Mechanics of Materials
- Engineering(all)
- Mechanical Engineering
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In: Materials Science and Engineering C, Vol. 41, 01.08.2014, p. 267-273.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Novel magnesium alloy Mg-2La caused no cytotoxic effects on cells in physiological conditions
AU - Weizbauer, Andreas
AU - Seitz, Jan Marten
AU - Werle, Peter
AU - Hegermann, Jan
AU - Willbold, Elmar
AU - Eifler, Rainer
AU - Windhagen, Henning
AU - Reifenrath, Janin
AU - Waizy, Hazibullah
N1 - Funding information: The authors gratefully acknowledge the financial support given by the German Research Foundation (DFG) within the collaborative research project SFB 599 . We thank Dr. Tomas Correa and Dr. Renate Weizbauer for valuable help with language accuracy and Dr. Anneke Loos and Dr. Christina Reufsteck for excellent technical support.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to < 70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.
AB - Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to < 70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.
KW - Cytotoxicity
KW - Degradation
KW - In vitro
KW - Magnesium alloy
KW - Orthopedic
UR - http://www.scopus.com/inward/record.url?scp=84901064469&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2014.04.063
DO - 10.1016/j.msec.2014.04.063
M3 - Article
C2 - 24907760
AN - SCOPUS:84901064469
VL - 41
SP - 267
EP - 273
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -