Details
| Original language | English |
|---|---|
| Pages (from-to) | 5-9 |
| Number of pages | 5 |
| Journal | Current Directions in Biomedical Engineering |
| Volume | 2 |
| Issue number | 1 |
| Early online date | 30 Sept 2016 |
| Publication status | Published - Sept 2016 |
Abstract
A major challenge in cardiovascular tissue engineering is the fabrication of scaffolds, which provide appropriate morphological and mechanical properties while avoiding undesirable immune reactions. In this study electrospinning was used to fabricate scaffolds out of blood proteins for cardiovascular tissue engineering. Lyophilised porcine plasma was dissolved in deionised water at a final concentration of 7.5% m/v and blended with 3.7% m/v PEO. Electrospinning resulted in homogeneous fibre morphologies with a mean fibre diameter of 151 nm, which could be adapted to create macroscopic shapes (mats, tubes). Cross-linking with glutaraldehyde vapour improved the long-term stability of protein based scaffolds in comparison to untreated scaffolds, resulting in a mass loss of 41% and 96% after 28 days of incubation in aqueous solution, respectively.
Keywords
- Biopolymer solution, Biostability analysis, Blood protein based scaffold, Cross-linking, Electrospinning, Glutaraldehyde vapour, Long-term stability, Tissue engineering
ASJC Scopus subject areas
- Engineering(all)
- Biomedical Engineering
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In: Current Directions in Biomedical Engineering, Vol. 2, No. 1, 09.2016, p. 5-9.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Novel blood protein based scaffolds for cardiovascular tissue engineering
AU - Kuhn, Antonia I.
AU - Müller, Marc
AU - Knigge, Sara
AU - Glasmacher, Birgit
N1 - Funding Information: Research funding: This work was kindly financially supported by the German Research Foundation (REBIRTH, EXC 62/1). Conflict of interest: Authors state no conflict of interest. Material and Methods: Informed consent: Informed consent has been obtained from all individuals included in this study. Ethical approval: The research related to human use complies with all the relevant national regulations, institutional policies and was performed in accordance with the tenets of the Helsinki Declaration, and has been approved by the authors’ institutional review board or equivalent committee.
PY - 2016/9
Y1 - 2016/9
N2 - A major challenge in cardiovascular tissue engineering is the fabrication of scaffolds, which provide appropriate morphological and mechanical properties while avoiding undesirable immune reactions. In this study electrospinning was used to fabricate scaffolds out of blood proteins for cardiovascular tissue engineering. Lyophilised porcine plasma was dissolved in deionised water at a final concentration of 7.5% m/v and blended with 3.7% m/v PEO. Electrospinning resulted in homogeneous fibre morphologies with a mean fibre diameter of 151 nm, which could be adapted to create macroscopic shapes (mats, tubes). Cross-linking with glutaraldehyde vapour improved the long-term stability of protein based scaffolds in comparison to untreated scaffolds, resulting in a mass loss of 41% and 96% after 28 days of incubation in aqueous solution, respectively.
AB - A major challenge in cardiovascular tissue engineering is the fabrication of scaffolds, which provide appropriate morphological and mechanical properties while avoiding undesirable immune reactions. In this study electrospinning was used to fabricate scaffolds out of blood proteins for cardiovascular tissue engineering. Lyophilised porcine plasma was dissolved in deionised water at a final concentration of 7.5% m/v and blended with 3.7% m/v PEO. Electrospinning resulted in homogeneous fibre morphologies with a mean fibre diameter of 151 nm, which could be adapted to create macroscopic shapes (mats, tubes). Cross-linking with glutaraldehyde vapour improved the long-term stability of protein based scaffolds in comparison to untreated scaffolds, resulting in a mass loss of 41% and 96% after 28 days of incubation in aqueous solution, respectively.
KW - Biopolymer solution
KW - Biostability analysis
KW - Blood protein based scaffold
KW - Cross-linking
KW - Electrospinning
KW - Glutaraldehyde vapour
KW - Long-term stability
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85059820837&partnerID=8YFLogxK
U2 - 10.1515/cdbme-2016-0005
DO - 10.1515/cdbme-2016-0005
M3 - Article
AN - SCOPUS:85059820837
VL - 2
SP - 5
EP - 9
JO - Current Directions in Biomedical Engineering
JF - Current Directions in Biomedical Engineering
IS - 1
ER -