Details
| Original language | English |
|---|---|
| Title of host publication | Biotechnology of Isoprenoids |
| Place of Publication | Cham |
| Publisher | Springer Verlag |
| Pages | 391-404 |
| Number of pages | 14 |
| ISBN (electronic) | 9783319201078 |
| ISBN (print) | 9783319201061 |
| Publication status | Published - 19 Oct 2014 |
Publication series
| Name | Advances in Biochemical Engineering/Biotechnology |
|---|---|
| Publisher | Springer Science and Business Media Deutschland GmbH |
| ISSN (Print) | 0724-6145 |
Abstract
The continuing interest in the sesquiterpene ketone (+)-nootkatone is stimulated by its strong grapefruit-like odor and numerous further bioactivities. Also numerous were the attempts to chemosynthesize or biotechnologically produce the compound. Cytochrome P450 enzymes from bacteria and fungi were intensively studied and expressed in Escherichia coli and in more food compatible hosts, such as Saccharomyces cerevisiae. The lipoxygenase-catalyzed generation was demonstrated using an enzyme from several Pleurotus species. Laccases required artificial mediators for an efficient catalysis. More recently, plant valencene synthases were expressed in microbial hosts. Combined with an endogenous farnesyl diphosphate delivery pathway and a valencene oxidase, this approach opened access to high yields of nootkatone possessing the appreciated attribute of “natural” according to present food legislation. Little biochemical engineering was carried out on the novel recombinant strains, leaving many options for future improved bioprocesses.
Keywords
- CYP450, Insecticide, Laccase, Lipoxygenase, Natural flavor, Valencene oxidase, Valencene synthase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
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- Apa
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Biotechnology of Isoprenoids. Cham: Springer Verlag, 2014. p. 391-404 (Advances in Biochemical Engineering/Biotechnology).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Nootkatone
AU - Leonhardt, Robin Hagen
AU - Berger, Ralf G.
N1 - Funding information: RGB thanks his group members for their contributions to the work included in this chapter. Financial support came from the Deutsche Bundesstiftung Umwelt (ChemBioTec AZ13187), Forschungskreis der Ernährungsindustrie via AiF and BMWi (299 ZN) and the BMBF cluster Biokatalyse 2021 (FKZ0315172B).
PY - 2014/10/19
Y1 - 2014/10/19
N2 - The continuing interest in the sesquiterpene ketone (+)-nootkatone is stimulated by its strong grapefruit-like odor and numerous further bioactivities. Also numerous were the attempts to chemosynthesize or biotechnologically produce the compound. Cytochrome P450 enzymes from bacteria and fungi were intensively studied and expressed in Escherichia coli and in more food compatible hosts, such as Saccharomyces cerevisiae. The lipoxygenase-catalyzed generation was demonstrated using an enzyme from several Pleurotus species. Laccases required artificial mediators for an efficient catalysis. More recently, plant valencene synthases were expressed in microbial hosts. Combined with an endogenous farnesyl diphosphate delivery pathway and a valencene oxidase, this approach opened access to high yields of nootkatone possessing the appreciated attribute of “natural” according to present food legislation. Little biochemical engineering was carried out on the novel recombinant strains, leaving many options for future improved bioprocesses.
AB - The continuing interest in the sesquiterpene ketone (+)-nootkatone is stimulated by its strong grapefruit-like odor and numerous further bioactivities. Also numerous were the attempts to chemosynthesize or biotechnologically produce the compound. Cytochrome P450 enzymes from bacteria and fungi were intensively studied and expressed in Escherichia coli and in more food compatible hosts, such as Saccharomyces cerevisiae. The lipoxygenase-catalyzed generation was demonstrated using an enzyme from several Pleurotus species. Laccases required artificial mediators for an efficient catalysis. More recently, plant valencene synthases were expressed in microbial hosts. Combined with an endogenous farnesyl diphosphate delivery pathway and a valencene oxidase, this approach opened access to high yields of nootkatone possessing the appreciated attribute of “natural” according to present food legislation. Little biochemical engineering was carried out on the novel recombinant strains, leaving many options for future improved bioprocesses.
KW - CYP450
KW - Insecticide
KW - Laccase
KW - Lipoxygenase
KW - Natural flavor
KW - Valencene oxidase
KW - Valencene synthase
UR - http://www.scopus.com/inward/record.url?scp=84930225646&partnerID=8YFLogxK
U2 - 10.1007/10_2014_279
DO - 10.1007/10_2014_279
M3 - Contribution to book/anthology
C2 - 25326849
AN - SCOPUS:84930225646
SN - 9783319201061
T3 - Advances in Biochemical Engineering/Biotechnology
SP - 391
EP - 404
BT - Biotechnology of Isoprenoids
PB - Springer Verlag
CY - Cham
ER -