Details
| Original language | English |
|---|---|
| Pages (from-to) | 73-77 |
| Number of pages | 5 |
| Journal | BioNanoMaterials |
| Volume | 17 |
| Issue number | 1-2 |
| Early online date | 18 Apr 2016 |
| Publication status | Published - 1 May 2016 |
Abstract
In this letter we report a fast and easy method which could be used for initial screening of multispecies-biofilm development on putative new dental implant materials. Most staining methods require numerous washing steps that can result in detachment of loosely bound biofilms and therefore falsify the results. Thus, we used glutaraldehyde fixation, which induces autofluorescence through bacterial membrane protein cross-linking and concurrently stabilizes the biofilm structure. We analyzed the biofilms with nonlinear laser scanning microscopy and were able to (I) evaluate the multispecies-biofilm growth and (II) distinguish between bacterial species based on different two-photon autofluorescence intensities.
Keywords
- autofluorescence, dental implant, glutaraldehyde, multiphoton microscopy, periimplantitis, Schiff base
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: BioNanoMaterials, Vol. 17, No. 1-2, 01.05.2016, p. 73-77.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Nonlinear laser scanning microscopy of oral multispecies-biofilms
T2 - Fixative induced fluorescence as a fast and economical in vitro screening method
AU - Andric, Nadine
AU - Ehmke, Tobias
AU - Stumpp, Nico S.
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
AU - Stiesch, Meike
N1 - This work was carried out as an integral part of the BIOFABRICATION FOR NIFE Initiative, which is financially supported by the ministry of Lower Saxony and the VolkswagenStiftung. NIFE is the Lower Saxony Center for Biomedical Engineering, Implant Research and Development, a joint translational research centre of the Hannover Medical School, the Leibniz University Hannover, the University of Veterinary Medicine Hannover and the Laser Zentrum Hannover e. V. Funding: Ministry of Lower Saxony and VolkswagenStiftung (both BIOFABRICATION FOR NIFE: VWZN2860).
PY - 2016/5/1
Y1 - 2016/5/1
N2 - In this letter we report a fast and easy method which could be used for initial screening of multispecies-biofilm development on putative new dental implant materials. Most staining methods require numerous washing steps that can result in detachment of loosely bound biofilms and therefore falsify the results. Thus, we used glutaraldehyde fixation, which induces autofluorescence through bacterial membrane protein cross-linking and concurrently stabilizes the biofilm structure. We analyzed the biofilms with nonlinear laser scanning microscopy and were able to (I) evaluate the multispecies-biofilm growth and (II) distinguish between bacterial species based on different two-photon autofluorescence intensities.
AB - In this letter we report a fast and easy method which could be used for initial screening of multispecies-biofilm development on putative new dental implant materials. Most staining methods require numerous washing steps that can result in detachment of loosely bound biofilms and therefore falsify the results. Thus, we used glutaraldehyde fixation, which induces autofluorescence through bacterial membrane protein cross-linking and concurrently stabilizes the biofilm structure. We analyzed the biofilms with nonlinear laser scanning microscopy and were able to (I) evaluate the multispecies-biofilm growth and (II) distinguish between bacterial species based on different two-photon autofluorescence intensities.
KW - autofluorescence
KW - dental implant
KW - glutaraldehyde
KW - multiphoton microscopy
KW - periimplantitis
KW - Schiff base
UR - http://www.scopus.com/inward/record.url?scp=84991518382&partnerID=8YFLogxK
U2 - 10.1515/bnm-2015-0028
DO - 10.1515/bnm-2015-0028
M3 - Article
AN - SCOPUS:84991518382
VL - 17
SP - 73
EP - 77
JO - BioNanoMaterials
JF - BioNanoMaterials
SN - 2193-0651
IS - 1-2
ER -