Details
| Original language | English |
|---|---|
| Pages (from-to) | 582-585 |
| Number of pages | 4 |
| Journal | Procedia Engineering |
| Volume | 168 |
| Publication status | Published - 4 Jan 2017 |
| Event | 30th Eurosensors Conference, Eurosensors 2016 - Budapest, Hungary Duration: 4 Sept 2016 → 7 Sept 2016 |
Abstract
To ensure high quality output of biotechnological processes, relevant process parameters need to be monitored. As bioprocesses are increasingly executed in single use bioreactors, there is an increasing demand for new sensors applicable to these processes. In this work, we present a low-cost sensor system for continuous non-invasive cell growth monitoring, especially for single use bioreactor applications. The system consists of a planar coil connected to a low cost network analyzer. The coil is attached to the outside of the polymer foil of the single use bioreactor and an impedance spectrum is measured. To evaluate the sensor, E. coli cultivations are performed in a modified cultivation setup, which enables measurements through the polymer foil of a Sartorius BIOSTAT® CultiBag RM, and additionally allows sampling of culture medium for optical density reference measurements. The resonance peak of the coil in the impedance spectrum, is observed as measure for the optical density. Regardless of the simple sensor construction, we found a good correlation between optical density and the damping ratio of the resonance peak.
Keywords
- cell growth monitoring, dielectric spectroscopy, inductive permittivity measurements, single use bioreactor
ASJC Scopus subject areas
- Engineering(all)
- General Engineering
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In: Procedia Engineering, Vol. 168, 04.01.2017, p. 582-585.
Research output: Contribution to journal › Conference article › Research › peer review
}
TY - JOUR
T1 - Non-Invasive Online Monitoring of Cell Growth in Disposable Bioreactors with a Planar Coil
AU - Reinecke, T.
AU - Biechele, P.
AU - Frickhöffer, M.
AU - Scheper, T.
AU - Zimmermann, S.
PY - 2017/1/4
Y1 - 2017/1/4
N2 - To ensure high quality output of biotechnological processes, relevant process parameters need to be monitored. As bioprocesses are increasingly executed in single use bioreactors, there is an increasing demand for new sensors applicable to these processes. In this work, we present a low-cost sensor system for continuous non-invasive cell growth monitoring, especially for single use bioreactor applications. The system consists of a planar coil connected to a low cost network analyzer. The coil is attached to the outside of the polymer foil of the single use bioreactor and an impedance spectrum is measured. To evaluate the sensor, E. coli cultivations are performed in a modified cultivation setup, which enables measurements through the polymer foil of a Sartorius BIOSTAT® CultiBag RM, and additionally allows sampling of culture medium for optical density reference measurements. The resonance peak of the coil in the impedance spectrum, is observed as measure for the optical density. Regardless of the simple sensor construction, we found a good correlation between optical density and the damping ratio of the resonance peak.
AB - To ensure high quality output of biotechnological processes, relevant process parameters need to be monitored. As bioprocesses are increasingly executed in single use bioreactors, there is an increasing demand for new sensors applicable to these processes. In this work, we present a low-cost sensor system for continuous non-invasive cell growth monitoring, especially for single use bioreactor applications. The system consists of a planar coil connected to a low cost network analyzer. The coil is attached to the outside of the polymer foil of the single use bioreactor and an impedance spectrum is measured. To evaluate the sensor, E. coli cultivations are performed in a modified cultivation setup, which enables measurements through the polymer foil of a Sartorius BIOSTAT® CultiBag RM, and additionally allows sampling of culture medium for optical density reference measurements. The resonance peak of the coil in the impedance spectrum, is observed as measure for the optical density. Regardless of the simple sensor construction, we found a good correlation between optical density and the damping ratio of the resonance peak.
KW - cell growth monitoring
KW - dielectric spectroscopy
KW - inductive permittivity measurements
KW - single use bioreactor
UR - http://www.scopus.com/inward/record.url?scp=85010006271&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2016.11.219
DO - 10.1016/j.proeng.2016.11.219
M3 - Conference article
AN - SCOPUS:85010006271
VL - 168
SP - 582
EP - 585
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
T2 - 30th Eurosensors Conference, Eurosensors 2016
Y2 - 4 September 2016 through 7 September 2016
ER -