Details
| Original language | English |
|---|---|
| Pages (from-to) | 321-326 |
| Number of pages | 6 |
| Journal | Journal of clinical periodontology |
| Volume | 41 |
| Issue number | 4 |
| Publication status | Published - 7 Jan 2014 |
Abstract
Aim Imaging with Confocal Laser Scanning Microscopy (CLSM) generates high-resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800-1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.
Keywords
- confocal laser scanning microscopy, gingivitis, in vivo imaging
ASJC Scopus subject areas
- Dentistry(all)
- Periodontics
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In: Journal of clinical periodontology, Vol. 41, No. 4, 07.01.2014, p. 321-326.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition
AU - Eberhard, Jörg
AU - Loewen, Hendrik
AU - Krüger, Alexander
AU - Donner, Sabine
AU - Stumpp, Nico
AU - Patzlaff, Mandy
AU - Stachs, Oliver
AU - Reichard, Maria
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
AU - Stiesch, Meike
PY - 2014/1/7
Y1 - 2014/1/7
N2 - Aim Imaging with Confocal Laser Scanning Microscopy (CLSM) generates high-resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800-1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.
AB - Aim Imaging with Confocal Laser Scanning Microscopy (CLSM) generates high-resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800-1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.
KW - confocal laser scanning microscopy
KW - gingivitis
KW - in vivo imaging
UR - http://www.scopus.com/inward/record.url?scp=84897647225&partnerID=8YFLogxK
U2 - 10.1111/jcpe.12227
DO - 10.1111/jcpe.12227
M3 - Article
C2 - 24393067
AN - SCOPUS:84897647225
VL - 41
SP - 321
EP - 326
JO - Journal of clinical periodontology
JF - Journal of clinical periodontology
SN - 0303-6979
IS - 4
ER -