Details
| Original language | English |
|---|---|
| Pages (from-to) | 75527-75535 |
| Number of pages | 9 |
| Journal | IEEE ACCESS |
| Volume | 10 |
| Publication status | Published - 19 Jul 2022 |
Abstract
Rheology serves to measure the deformation and flow of materials. Its associated quantities, for example, the Young's modulus, shear modulus, bulk modulus, or longitudinal modulus, are important in the biomedical field, in particular for soft materials, to characterize the response of materials to external force. Usually, mechanical probes, in particular rheometers or atomic force microscopy, are used to characterize these quantities. In the last decade, optical measurements have been derived to obtain these quantities even in small sample volumes. However, usually only one quantity is evaluated using optical techniques, such as Brillouin microscopy, which does not allow a full rheological characterization. The latter requires measuring at least two quantities, which allows for calculating all further rheological properties. In this paper, we aim to close this gap by combining two optical rheology methods, Brillouin microscopy to measure the longitudinal modulus and Laser Speckle Rheology for the shear modulus. We built an optical setup that allows the non-contact and hence non-destructive and non-invasive measurement of both quantities simultaneously in the same sample using a 780 nm, narrow linewidth (50 kHz) laser system. We evaluate our approach using defined samples of glycerol and polydimethylsiloxane and we demonstrate image acquisition using the combined setup. We also investigate porcine corneae, as biological samples, and demonstrate direct measurement of longitudinal modulus and shear modulus and calculation of Young's modulus, bulk modulus, and the Poission ratio, which are all in good agreement with published quantities. In the future, our approach allows for full characterization of the rheology of biological specimens.
Keywords
- Biomechanics, Brillouin scattering, cornea, rheology, speckle
ASJC Scopus subject areas
- Computer Science(all)
- General Computer Science
- Materials Science(all)
- General Materials Science
- Engineering(all)
- General Engineering
- Engineering(all)
- Electrical and Electronic Engineering
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In: IEEE ACCESS, Vol. 10, 19.07.2022, p. 75527-75535.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Non-Invasive Full Rheological Characterization via Combined Speckle and Brillouin Microscopy
AU - Wenzel, Johannes
AU - Rüprich, Niklas
AU - Sperlich, Karsten
AU - Stachs, Oliver
AU - Schunemann, Melanie
AU - Leyh, Colette
AU - Kalies, Stefan
AU - Heisterkamp, Alexander
N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (DFG) under Project 405601114.
PY - 2022/7/19
Y1 - 2022/7/19
N2 - Rheology serves to measure the deformation and flow of materials. Its associated quantities, for example, the Young's modulus, shear modulus, bulk modulus, or longitudinal modulus, are important in the biomedical field, in particular for soft materials, to characterize the response of materials to external force. Usually, mechanical probes, in particular rheometers or atomic force microscopy, are used to characterize these quantities. In the last decade, optical measurements have been derived to obtain these quantities even in small sample volumes. However, usually only one quantity is evaluated using optical techniques, such as Brillouin microscopy, which does not allow a full rheological characterization. The latter requires measuring at least two quantities, which allows for calculating all further rheological properties. In this paper, we aim to close this gap by combining two optical rheology methods, Brillouin microscopy to measure the longitudinal modulus and Laser Speckle Rheology for the shear modulus. We built an optical setup that allows the non-contact and hence non-destructive and non-invasive measurement of both quantities simultaneously in the same sample using a 780 nm, narrow linewidth (50 kHz) laser system. We evaluate our approach using defined samples of glycerol and polydimethylsiloxane and we demonstrate image acquisition using the combined setup. We also investigate porcine corneae, as biological samples, and demonstrate direct measurement of longitudinal modulus and shear modulus and calculation of Young's modulus, bulk modulus, and the Poission ratio, which are all in good agreement with published quantities. In the future, our approach allows for full characterization of the rheology of biological specimens.
AB - Rheology serves to measure the deformation and flow of materials. Its associated quantities, for example, the Young's modulus, shear modulus, bulk modulus, or longitudinal modulus, are important in the biomedical field, in particular for soft materials, to characterize the response of materials to external force. Usually, mechanical probes, in particular rheometers or atomic force microscopy, are used to characterize these quantities. In the last decade, optical measurements have been derived to obtain these quantities even in small sample volumes. However, usually only one quantity is evaluated using optical techniques, such as Brillouin microscopy, which does not allow a full rheological characterization. The latter requires measuring at least two quantities, which allows for calculating all further rheological properties. In this paper, we aim to close this gap by combining two optical rheology methods, Brillouin microscopy to measure the longitudinal modulus and Laser Speckle Rheology for the shear modulus. We built an optical setup that allows the non-contact and hence non-destructive and non-invasive measurement of both quantities simultaneously in the same sample using a 780 nm, narrow linewidth (50 kHz) laser system. We evaluate our approach using defined samples of glycerol and polydimethylsiloxane and we demonstrate image acquisition using the combined setup. We also investigate porcine corneae, as biological samples, and demonstrate direct measurement of longitudinal modulus and shear modulus and calculation of Young's modulus, bulk modulus, and the Poission ratio, which are all in good agreement with published quantities. In the future, our approach allows for full characterization of the rheology of biological specimens.
KW - Biomechanics
KW - Brillouin scattering
KW - cornea
KW - rheology
KW - speckle
UR - http://www.scopus.com/inward/record.url?scp=85135230199&partnerID=8YFLogxK
U2 - 10.1109/ACCESS.2022.3192463
DO - 10.1109/ACCESS.2022.3192463
M3 - Article
AN - SCOPUS:85135230199
VL - 10
SP - 75527
EP - 75535
JO - IEEE ACCESS
JF - IEEE ACCESS
SN - 2169-3536
ER -