Details
| Translated title of the contribution | Nonlinear Microscopy in Ophthalmology: Principles and Pathbreaking Applications |
|---|---|
| Original language | German |
| Pages (from-to) | 1365-1373 |
| Number of pages | 9 |
| Journal | Klinische Monatsblatter fur Augenheilkunde |
| Volume | 232 |
| Issue number | 12 |
| Publication status | Published - 17 Dec 2015 |
Abstract
Nonlinear microscopy is a non-invasive imaging technique which allows a visualization of biological tissue with high signal contrast due to spectral separation combined with high resolution. In addition to two-photon fluorescence and second harmonic signals also four-wave mixing signals can be used for imaging ocular structures. This review article presents the physical principles of different contrast mechanisms. Exemplary experimental results based on various nonlinear signals are shown, opportunities of this technology are discussed and the prospect of translating this imaging technique into a clinical application is addressed.
ASJC Scopus subject areas
- Medicine(all)
- Ophthalmology
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In: Klinische Monatsblatter fur Augenheilkunde, Vol. 232, No. 12, 17.12.2015, p. 1365-1373.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Nichtlineare Lasermikroskopie in der Ophthalmologie
T2 - Physikalische Prinzipien und wegweisende Anwendungen
AU - Ehmke, T.
AU - Krüger, Alexander
AU - Ripken, T.
AU - Rei, S.
AU - Stachs, O.
AU - Heisterkamp, Alexander
PY - 2015/12/17
Y1 - 2015/12/17
N2 - Nonlinear microscopy is a non-invasive imaging technique which allows a visualization of biological tissue with high signal contrast due to spectral separation combined with high resolution. In addition to two-photon fluorescence and second harmonic signals also four-wave mixing signals can be used for imaging ocular structures. This review article presents the physical principles of different contrast mechanisms. Exemplary experimental results based on various nonlinear signals are shown, opportunities of this technology are discussed and the prospect of translating this imaging technique into a clinical application is addressed.
AB - Nonlinear microscopy is a non-invasive imaging technique which allows a visualization of biological tissue with high signal contrast due to spectral separation combined with high resolution. In addition to two-photon fluorescence and second harmonic signals also four-wave mixing signals can be used for imaging ocular structures. This review article presents the physical principles of different contrast mechanisms. Exemplary experimental results based on various nonlinear signals are shown, opportunities of this technology are discussed and the prospect of translating this imaging technique into a clinical application is addressed.
KW - cornea
KW - four-wave mixing
KW - higher harmonics
KW - lens
KW - nonlinear microscopy
KW - two-photon fluorescence
UR - http://www.scopus.com/inward/record.url?scp=84950327534&partnerID=8YFLogxK
U2 - 10.1055/s-0041-109020
DO - 10.1055/s-0041-109020
M3 - Artikel
C2 - 26678898
AN - SCOPUS:84950327534
VL - 232
SP - 1365
EP - 1373
JO - Klinische Monatsblatter fur Augenheilkunde
JF - Klinische Monatsblatter fur Augenheilkunde
SN - 0023-2165
IS - 12
ER -