Details
| Original language | English |
|---|---|
| Article number | 102625 |
| Journal | Algal Research |
| Volume | 62 |
| Early online date | 10 Jan 2022 |
| Publication status | Published - Mar 2022 |
Abstract
With escalating urbanization and ever-increasing population, plastic pollution has become the problem of hour. Owing to its deep roots in our daily life, it is imperative to find an alternative. Sustainable production of poly hydroxy butyrate (PHB) can be our way out from this soup of plastic pollution. Microalgae, being photoautotrophs, helps in bioremediation and can metabolize the waste products into biodiesel, bioethanol, protein, and also bioplastics in the form of PHB. The present study reports the accumulation of PHB in Coelastrella sp., Ettlia texensis and Pecatinodesmus sp. isolated from nearby regions of Indore under nutrient stress and mixotrophic conditions. The individual effect of nutrients was studied, and concluded that under mixotrophic cultivation, Coelastrella sp. can accumulate up to 151.8 ± 12.1 μg/mg dry cell weight (DCW) of PHB under N1.5P0.04Ga10 supplementation in 72 h of cultivation. A two-stage cultivation strategy enabled the accumulation of high PHB content in microalgae without compromising the microalgal growth, which was noted in terms of chlorophyll and biomass accumulation. Principle component analysis (PCA) revealed close relation between biomass and poly hydroxybutyrate (PHB) accumulation with organic carbon presence having a great impact. These results pave the way for sustainable PHA/PHB production using three microalgal species.
Keywords
- ANOVA, Lipid, Microalgae, PHB, Principle component analysis, Protein
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
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In: Algal Research, Vol. 62, 102625, 03.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Newly isolated native microalgal strains producing polyhydroxybutyrate and energy storage precursors simultaneously
T2 - Targeting microalgal biorefinery
AU - Samadhiya, Kanchan
AU - Ghosh, Atreyee
AU - Nogueira, Regina
AU - Bala, Kiran
N1 - Funding Information: The authors are thankful to DAAD for the LUH-IITI mobility grant and IIT Indore for providing the necessary support. KS is thankful to MHRD, India for fellowship support. The funding agency has not played any role in the design or decisions regarding the publication of a manuscript. Authors are also thankful to Sophisticated Instruments Facility (SIC), IIT Indore for instrumentation facilities provided. Authors also acknowledge support from NCIM, Pune for identification services.
PY - 2022/3
Y1 - 2022/3
N2 - With escalating urbanization and ever-increasing population, plastic pollution has become the problem of hour. Owing to its deep roots in our daily life, it is imperative to find an alternative. Sustainable production of poly hydroxy butyrate (PHB) can be our way out from this soup of plastic pollution. Microalgae, being photoautotrophs, helps in bioremediation and can metabolize the waste products into biodiesel, bioethanol, protein, and also bioplastics in the form of PHB. The present study reports the accumulation of PHB in Coelastrella sp., Ettlia texensis and Pecatinodesmus sp. isolated from nearby regions of Indore under nutrient stress and mixotrophic conditions. The individual effect of nutrients was studied, and concluded that under mixotrophic cultivation, Coelastrella sp. can accumulate up to 151.8 ± 12.1 μg/mg dry cell weight (DCW) of PHB under N1.5P0.04Ga10 supplementation in 72 h of cultivation. A two-stage cultivation strategy enabled the accumulation of high PHB content in microalgae without compromising the microalgal growth, which was noted in terms of chlorophyll and biomass accumulation. Principle component analysis (PCA) revealed close relation between biomass and poly hydroxybutyrate (PHB) accumulation with organic carbon presence having a great impact. These results pave the way for sustainable PHA/PHB production using three microalgal species.
AB - With escalating urbanization and ever-increasing population, plastic pollution has become the problem of hour. Owing to its deep roots in our daily life, it is imperative to find an alternative. Sustainable production of poly hydroxy butyrate (PHB) can be our way out from this soup of plastic pollution. Microalgae, being photoautotrophs, helps in bioremediation and can metabolize the waste products into biodiesel, bioethanol, protein, and also bioplastics in the form of PHB. The present study reports the accumulation of PHB in Coelastrella sp., Ettlia texensis and Pecatinodesmus sp. isolated from nearby regions of Indore under nutrient stress and mixotrophic conditions. The individual effect of nutrients was studied, and concluded that under mixotrophic cultivation, Coelastrella sp. can accumulate up to 151.8 ± 12.1 μg/mg dry cell weight (DCW) of PHB under N1.5P0.04Ga10 supplementation in 72 h of cultivation. A two-stage cultivation strategy enabled the accumulation of high PHB content in microalgae without compromising the microalgal growth, which was noted in terms of chlorophyll and biomass accumulation. Principle component analysis (PCA) revealed close relation between biomass and poly hydroxybutyrate (PHB) accumulation with organic carbon presence having a great impact. These results pave the way for sustainable PHA/PHB production using three microalgal species.
KW - ANOVA
KW - Lipid
KW - Microalgae
KW - PHB
KW - Principle component analysis
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=85122616915&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2021.102625
DO - 10.1016/j.algal.2021.102625
M3 - Article
AN - SCOPUS:85122616915
VL - 62
JO - Algal Research
JF - Algal Research
SN - 2211-9264
M1 - 102625
ER -