Details
| Original language | English |
|---|---|
| Pages (from-to) | 1035-1043 |
| Number of pages | 9 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 221 |
| Publication status | Published - 18 Jul 2015 |
Abstract
Abstract Online pH control during microbial shake flask cultivation has not been established due to the lack of a practical combination of an online sensor system and an appropriate control unit. The objective of this investigation was to develop a minimum scale dosage apparatus, namely shake flask controller ("SFC"), which can control the pH during a complete cultivation and serves as technical example for the application of small liquid dispensing lab devices. A well evaluated optical, chemosensor based, noninvasive, multisensory platform prototype for online DO (dissolved oxygen)-, pH- and biomass measurement served as sensor. The SFC was designed as cap-integrated, semi-autarkical control unit. Minimum scale working parts like the commercial mp6 piezoelectric micropumps and miniature solenoid valves were combined with a selective laser sintering (SLS) printed backbone. In general it is intended to extend its application range on the control of enzymatic assays, polymerization processes, cell disruption methods or the precise dispense of special chemicals like inducers or inhibitors. It could be proved that pH control within a range of 0.1 pH units could be maintained at different cultivation conditions. A proportional-integral-derivative- (PID) controller and an adaptive proportional controller were successfully applied to calculate the balancing solution volume. SLS based 3D printing using polyamide combined with state-of-the-art micro pumps proved to be perfectly adaptable for minimum size, autoclavable lab devices.
Keywords
- 3D-printing, pH-control, pH-monitoring, Piezo pump, Shake flask
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
- Materials Science(all)
- Materials Chemistry
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In: Sensors and Actuators, B: Chemical, Vol. 221, 18.07.2015, p. 1035-1043.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - New perspectives in shake flask pH control using a 3D-printed control unit based on pH online measurement
AU - Ude, Christian
AU - Hentrop, Thorleif
AU - Lindner, Patrick
AU - Lücking, Tim H.
AU - Scheper, Thomas
AU - Beutel, Sascha
N1 - Funding information: We would like to thank all members of our research groups for providing feedback and suggestions. This project was supported by the BMWi via AiF project within the ZIM-initiative (KF2407209FR2).
PY - 2015/7/18
Y1 - 2015/7/18
N2 - Abstract Online pH control during microbial shake flask cultivation has not been established due to the lack of a practical combination of an online sensor system and an appropriate control unit. The objective of this investigation was to develop a minimum scale dosage apparatus, namely shake flask controller ("SFC"), which can control the pH during a complete cultivation and serves as technical example for the application of small liquid dispensing lab devices. A well evaluated optical, chemosensor based, noninvasive, multisensory platform prototype for online DO (dissolved oxygen)-, pH- and biomass measurement served as sensor. The SFC was designed as cap-integrated, semi-autarkical control unit. Minimum scale working parts like the commercial mp6 piezoelectric micropumps and miniature solenoid valves were combined with a selective laser sintering (SLS) printed backbone. In general it is intended to extend its application range on the control of enzymatic assays, polymerization processes, cell disruption methods or the precise dispense of special chemicals like inducers or inhibitors. It could be proved that pH control within a range of 0.1 pH units could be maintained at different cultivation conditions. A proportional-integral-derivative- (PID) controller and an adaptive proportional controller were successfully applied to calculate the balancing solution volume. SLS based 3D printing using polyamide combined with state-of-the-art micro pumps proved to be perfectly adaptable for minimum size, autoclavable lab devices.
AB - Abstract Online pH control during microbial shake flask cultivation has not been established due to the lack of a practical combination of an online sensor system and an appropriate control unit. The objective of this investigation was to develop a minimum scale dosage apparatus, namely shake flask controller ("SFC"), which can control the pH during a complete cultivation and serves as technical example for the application of small liquid dispensing lab devices. A well evaluated optical, chemosensor based, noninvasive, multisensory platform prototype for online DO (dissolved oxygen)-, pH- and biomass measurement served as sensor. The SFC was designed as cap-integrated, semi-autarkical control unit. Minimum scale working parts like the commercial mp6 piezoelectric micropumps and miniature solenoid valves were combined with a selective laser sintering (SLS) printed backbone. In general it is intended to extend its application range on the control of enzymatic assays, polymerization processes, cell disruption methods or the precise dispense of special chemicals like inducers or inhibitors. It could be proved that pH control within a range of 0.1 pH units could be maintained at different cultivation conditions. A proportional-integral-derivative- (PID) controller and an adaptive proportional controller were successfully applied to calculate the balancing solution volume. SLS based 3D printing using polyamide combined with state-of-the-art micro pumps proved to be perfectly adaptable for minimum size, autoclavable lab devices.
KW - 3D-printing
KW - pH-control
KW - pH-monitoring
KW - Piezo pump
KW - Shake flask
UR - http://www.scopus.com/inward/record.url?scp=84938494237&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2015.07.017
DO - 10.1016/j.snb.2015.07.017
M3 - Article
AN - SCOPUS:84938494237
VL - 221
SP - 1035
EP - 1043
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -