Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the XXVI International Eucarpia Symposium Section Ornamentals: Editing Novelty |
| Subtitle of host publication | Erfurt, Germany, September 1-4, 2019 |
| Pages | 89-96 |
| Number of pages | 8 |
| Volume | 1283 |
| Publication status | Published - 3 Jul 2020 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Publisher | International Society for Horticultural Science |
| ISSN (Print) | 0567-7572 |
Abstract
The responses of plants to attacks by pathogenic fungi are controlled by a complex network of different signaling pathways. The successful recognition of the pathogen and the resulting signal processing up to the expression of the required defence genes can lead to resistant plants. This project deals with the interaction between roses and the fungal pathogens Diplocarpon rosae and Podosphaera pannosa, causing rose black spot disease and powdery mildew, respectively. This allows the comparison of infection strategies and differences caused by the different fungal lifestyle. So far, the resistance mechanisms and signaling pathways used are not fully known. Another complicating aspect is the fact that these signal transduction networks are susceptible to interference. It is not only the pathogen itself that specifically manipulates signaling pathways through effector proteins, but also abiotic environmental conditions that can influence them. This can have both positive and negative effects on the plant's defence response. Here we take a closer look at the influence of phosphate deficiency. Via qRT-PCR the expression of five phosphate starvation induced (PSI) genes was analysed for different treatments. These data were collected, together with controls, in three independent biological replicates and are available at three points in time. We found an interesting downregulation of the PSI genes in leaves infected with P. pannosa compared to controls and D. rosae infections. Between roses that are either resistant or susceptible to D. rosae, however, only few significant changes were observed. This could be a first indication of a role of the phosphate signaling pathway during infection with the pathogenic fungus P. pannosa and a possible link of abiotic and biotic stress response pathways.
Keywords
- Black spot, Crosstalk, Defence response, Phosphate starvation, Powdery mildew, Roses, Signaling
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the XXVI International Eucarpia Symposium Section Ornamentals: Editing Novelty: Erfurt, Germany, September 1-4, 2019 . Vol. 1283 2020. p. 89-96 (Acta Horticulturae).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - New insights into interactions of roses and pathogenic fungi and crosstalk potential of various stress signalling pathways
AU - Domes, Helena Sophia
AU - Debener, T.
N1 - Funding information: We want to give special thanks to Enzo Neu and Ina Menz who contributed so much to this experiment. Also, we want to thank the Institut für Rebenzüchtung of the Julius Kühn-Institut (JKI) in Siebeldingen. In particular, the group of Prof. Dr. Eva Zyprian for the support in performing high-throughput qPCR with the BioMark system. Additionally, we thank the DFG for the funding of this project in the background of the GRK 1798.
PY - 2020/7/3
Y1 - 2020/7/3
N2 - The responses of plants to attacks by pathogenic fungi are controlled by a complex network of different signaling pathways. The successful recognition of the pathogen and the resulting signal processing up to the expression of the required defence genes can lead to resistant plants. This project deals with the interaction between roses and the fungal pathogens Diplocarpon rosae and Podosphaera pannosa, causing rose black spot disease and powdery mildew, respectively. This allows the comparison of infection strategies and differences caused by the different fungal lifestyle. So far, the resistance mechanisms and signaling pathways used are not fully known. Another complicating aspect is the fact that these signal transduction networks are susceptible to interference. It is not only the pathogen itself that specifically manipulates signaling pathways through effector proteins, but also abiotic environmental conditions that can influence them. This can have both positive and negative effects on the plant's defence response. Here we take a closer look at the influence of phosphate deficiency. Via qRT-PCR the expression of five phosphate starvation induced (PSI) genes was analysed for different treatments. These data were collected, together with controls, in three independent biological replicates and are available at three points in time. We found an interesting downregulation of the PSI genes in leaves infected with P. pannosa compared to controls and D. rosae infections. Between roses that are either resistant or susceptible to D. rosae, however, only few significant changes were observed. This could be a first indication of a role of the phosphate signaling pathway during infection with the pathogenic fungus P. pannosa and a possible link of abiotic and biotic stress response pathways.
AB - The responses of plants to attacks by pathogenic fungi are controlled by a complex network of different signaling pathways. The successful recognition of the pathogen and the resulting signal processing up to the expression of the required defence genes can lead to resistant plants. This project deals with the interaction between roses and the fungal pathogens Diplocarpon rosae and Podosphaera pannosa, causing rose black spot disease and powdery mildew, respectively. This allows the comparison of infection strategies and differences caused by the different fungal lifestyle. So far, the resistance mechanisms and signaling pathways used are not fully known. Another complicating aspect is the fact that these signal transduction networks are susceptible to interference. It is not only the pathogen itself that specifically manipulates signaling pathways through effector proteins, but also abiotic environmental conditions that can influence them. This can have both positive and negative effects on the plant's defence response. Here we take a closer look at the influence of phosphate deficiency. Via qRT-PCR the expression of five phosphate starvation induced (PSI) genes was analysed for different treatments. These data were collected, together with controls, in three independent biological replicates and are available at three points in time. We found an interesting downregulation of the PSI genes in leaves infected with P. pannosa compared to controls and D. rosae infections. Between roses that are either resistant or susceptible to D. rosae, however, only few significant changes were observed. This could be a first indication of a role of the phosphate signaling pathway during infection with the pathogenic fungus P. pannosa and a possible link of abiotic and biotic stress response pathways.
KW - Black spot
KW - Crosstalk
KW - Defence response
KW - Phosphate starvation
KW - Powdery mildew
KW - Roses
KW - Signaling
UR - http://www.scopus.com/inward/record.url?scp=85089382522&partnerID=8YFLogxK
U2 - 10.17660/actahortic.2020.1283.13
DO - 10.17660/actahortic.2020.1283.13
M3 - Conference contribution
AN - SCOPUS:85089382522
SN - 978-94-62612-81-5
VL - 1283
T3 - Acta Horticulturae
SP - 89
EP - 96
BT - Proceedings of the XXVI International Eucarpia Symposium Section Ornamentals: Editing Novelty
ER -