Details
| Original language | English |
|---|---|
| Pages (from-to) | 496-508 |
| Number of pages | 13 |
| Journal | Systematics and biodiversity |
| Volume | 13 |
| Issue number | 5 |
| Publication status | Published - 3 Sept 2015 |
Abstract
Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as DNA barcodes. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.
Keywords
- genetic diversity, Halophila, ITS, matK, rbcL, seagrass
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Systematics and biodiversity, Vol. 13, No. 5, 03.09.2015, p. 496-508.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - New insights into DNA barcoding of seagrasses
AU - Nguyen, Xuan Vy
AU - Höfler, Saskia
AU - Glasenapp, Yvana
AU - Thangaradjou, Thirunavukarassu
AU - Lucas, Christina
AU - Papenbrock, Jutta
PY - 2015/9/3
Y1 - 2015/9/3
N2 - Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as DNA barcodes. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.
AB - Taxonomists find some plant genera challenging because of the few morphological differences or unclear characters among closely related species, which leads to the misidentification of taxa. DNA barcoding is an approach to identify species by using short orthologous DNA sequences, known as DNA barcodes. Concatenated rbcL and matK sequences are considered DNA barcodes for seagrasses. However, these markers are not applicable to all members of seagrasses at the species level, especially within the genus Halophila. Our previous studies indicated that the internal transcribed spacer (ITS) showed higher species resolution than the concatenated rbcL and matK sequences in the case of Halophila ovalis and closely related species. In this study, 26 ITS, two rbcL and two matK consensus sequences from 18 seagrass taxa belonging to four families collected in India, Vietnam, Germany, Croatia and Egypt were processed. Molecular ITS analysis resolved five clades. The results also indicate that the Cymodoceaceae family might be a non-monophyletic group. In conclusion, ITS could be applied as a DNA barcode for seagrasses instead of the rbcL/matK system previously proposed.
KW - genetic diversity
KW - Halophila
KW - ITS
KW - matK
KW - rbcL
KW - seagrass
UR - http://www.scopus.com/inward/record.url?scp=84938949963&partnerID=8YFLogxK
U2 - 10.1080/14772000.2015.1046408
DO - 10.1080/14772000.2015.1046408
M3 - Article
AN - SCOPUS:84938949963
VL - 13
SP - 496
EP - 508
JO - Systematics and biodiversity
JF - Systematics and biodiversity
SN - 1477-2000
IS - 5
ER -