Details
| Original language | English |
|---|---|
| Pages (from-to) | 151-162 |
| Number of pages | 12 |
| Journal | Acta Horticulturae |
| Volume | 751 |
| Publication status | Published - 2007 |
Abstract
Degenerate oligonucleotide primers targeting conserved motifs within the NBS region of nucleotide binding site (NBS)-leucine-rich repeat (LRR) resistance (R) genes were used to isolate resistance gene analogs (RGAs) from roses. A large RGA sublibrary consisting of 7000 clones was established. Sixty-seven percent of this sublibrary has been characterized and contains at least 40 unique RGA families of variable sizes, which could be subdivided further into the TIR (toll-/ interleukin-1 receptor) and the LZ (leucine zipper) group. TIR- and LZ-RGAs show a clear phylo- genetic separation, while distances between the single members of the LZ group are larger. The RGAs were organized as single-, low- and multicopy loci in the rose genome, but none of the analyzed sequences was conserved in Prunus cerasus. We studied the expression of some of the rose RGAs in a black spot resistant rose genotype and identified several candidates probably involved directly in the resistance reaction against black spot or in general stress responses. Finally, we integrated the RGAs into two different rose chromosome maps, which also contain the single dominant R genes Rdrl against black spot and Rppl against powDery mildew (PM) and several QTL (quantitative trait loci) involved in PM resistance. We identified some RGAs in chromosomal regions together with QTL for PM resistance as well as a number of RGA clusters probably indicating genomic regions containing not yet identified R genes.
Keywords
- Black spot, Evolution, Mapping, PowDery mildew, Resistance gene analogs (RGAs), Resistance genes
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Acta Horticulturae, Vol. 751, 2007, p. 151-162.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - NBS-LRR-RGAs in roses
T2 - Diversity, genomic organization, expression and chromosomal location
AU - Hattendorf, A.
AU - Debener, T.
PY - 2007
Y1 - 2007
N2 - Degenerate oligonucleotide primers targeting conserved motifs within the NBS region of nucleotide binding site (NBS)-leucine-rich repeat (LRR) resistance (R) genes were used to isolate resistance gene analogs (RGAs) from roses. A large RGA sublibrary consisting of 7000 clones was established. Sixty-seven percent of this sublibrary has been characterized and contains at least 40 unique RGA families of variable sizes, which could be subdivided further into the TIR (toll-/ interleukin-1 receptor) and the LZ (leucine zipper) group. TIR- and LZ-RGAs show a clear phylo- genetic separation, while distances between the single members of the LZ group are larger. The RGAs were organized as single-, low- and multicopy loci in the rose genome, but none of the analyzed sequences was conserved in Prunus cerasus. We studied the expression of some of the rose RGAs in a black spot resistant rose genotype and identified several candidates probably involved directly in the resistance reaction against black spot or in general stress responses. Finally, we integrated the RGAs into two different rose chromosome maps, which also contain the single dominant R genes Rdrl against black spot and Rppl against powDery mildew (PM) and several QTL (quantitative trait loci) involved in PM resistance. We identified some RGAs in chromosomal regions together with QTL for PM resistance as well as a number of RGA clusters probably indicating genomic regions containing not yet identified R genes.
AB - Degenerate oligonucleotide primers targeting conserved motifs within the NBS region of nucleotide binding site (NBS)-leucine-rich repeat (LRR) resistance (R) genes were used to isolate resistance gene analogs (RGAs) from roses. A large RGA sublibrary consisting of 7000 clones was established. Sixty-seven percent of this sublibrary has been characterized and contains at least 40 unique RGA families of variable sizes, which could be subdivided further into the TIR (toll-/ interleukin-1 receptor) and the LZ (leucine zipper) group. TIR- and LZ-RGAs show a clear phylo- genetic separation, while distances between the single members of the LZ group are larger. The RGAs were organized as single-, low- and multicopy loci in the rose genome, but none of the analyzed sequences was conserved in Prunus cerasus. We studied the expression of some of the rose RGAs in a black spot resistant rose genotype and identified several candidates probably involved directly in the resistance reaction against black spot or in general stress responses. Finally, we integrated the RGAs into two different rose chromosome maps, which also contain the single dominant R genes Rdrl against black spot and Rppl against powDery mildew (PM) and several QTL (quantitative trait loci) involved in PM resistance. We identified some RGAs in chromosomal regions together with QTL for PM resistance as well as a number of RGA clusters probably indicating genomic regions containing not yet identified R genes.
KW - Black spot
KW - Evolution
KW - Mapping
KW - PowDery mildew
KW - Resistance gene analogs (RGAs)
KW - Resistance genes
UR - http://www.scopus.com/inward/record.url?scp=70449447709&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2007.751.17
DO - 10.17660/ActaHortic.2007.751.17
M3 - Article
AN - SCOPUS:70449447709
VL - 751
SP - 151
EP - 162
JO - Acta Horticulturae
JF - Acta Horticulturae
SN - 0567-7572
ER -