Details
| Original language | English |
|---|---|
| Title of host publication | Optical Interactions with Tissue and Cells XVI |
| Subtitle of host publication | 22 - 26 January 2005, San Jose, California, USA |
| Place of Publication | Bellingham |
| Publisher | SPIE |
| Pages | 230-235 |
| Number of pages | 6 |
| ISBN (print) | 0-8194-5669-1 |
| Publication status | Published - 15 Apr 2005 |
| Externally published | Yes |
| Event | Optical Interactions with Tissue and Cells XVI - San Jose, CA, United States Duration: 24 Jan 2005 → 26 Jan 2005 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Publisher | SPIE |
| Volume | 5695 |
| ISSN (Print) | 1605-7422 |
Abstract
We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
Keywords
- Ablation, Actin, Biology, Cell surgery, Microtubule, Photodisruption, Ultrashort laser pulses
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- Apa
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- BibTeX
- RIS
Optical Interactions with Tissue and Cells XVI: 22 - 26 January 2005, San Jose, California, USA. Bellingham: SPIE, 2005. p. 230-235 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 5695).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Nanosurgery in live cells using ultrashort laser pulses
AU - Heisterkamp, A.
AU - Maxwell, I. Z.
AU - Kumar, Sanjay
AU - Underwood, Jean M.
AU - Nickerson, Jeffrey A.
AU - Ingber, D. E.
AU - Mazur, E.
PY - 2005/4/15
Y1 - 2005/4/15
N2 - We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
AB - We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
KW - Ablation
KW - Actin
KW - Biology
KW - Cell surgery
KW - Microtubule
KW - Photodisruption
KW - Ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=23244441634&partnerID=8YFLogxK
U2 - 10.1117/12.590467
DO - 10.1117/12.590467
M3 - Conference contribution
AN - SCOPUS:23244441634
SN - 0-8194-5669-1
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
SP - 230
EP - 235
BT - Optical Interactions with Tissue and Cells XVI
PB - SPIE
CY - Bellingham
T2 - Optical Interactions with Tissue and Cells XVI
Y2 - 24 January 2005 through 26 January 2005
ER -