Details
| Original language | English |
|---|---|
| Pages (from-to) | 3739-3756 |
| Number of pages | 18 |
| Journal | PLANT CELL |
| Volume | 35 |
| Issue number | 10 |
| Early online date | 27 Jun 2023 |
| Publication status | Published - Oct 2023 |
Abstract
The biological function of RNA can be modulated by base modifications. Here, we unveiled the occurrence of N4-acetylation of cytidine in plant RNA, including mRNA, by employing LC-MS/MS and acRIP-seq. We identified 325 acetylated transcripts from the leaves of 4-week-old Arabidopsis (Arabidopsis thaliana) plants and determined that 2 partially redundant N-ACETYLTRANSFERASEs FOR CYTIDINE IN RNA (ACYR1 and ACYR2), which are homologous to mammalian NAT10, are required for acetylating RNA in vivo. A double-null mutant was embryo lethal, while eliminating 3 of the 4 ACYR alleles led to defects in leaf development. These phenotypes could be traced back to the reduced acetylation and concomitant destabilization of the transcript of TOUGH, which is required for miRNA processing. These findings indicate that N4-acetylation of cytidine is a modulator of RNA function with a critical role in plant development and likely many other processes.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANT CELL, Vol. 35, No. 10, 10.2023, p. 3739-3756.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - N4-acetylation of cytidine in mRNA plays essential roles in plants
AU - Wang, Wenlei
AU - Liu, Huijie
AU - Wang, Feifei
AU - Liu, Xiaoye
AU - Sun, Yu
AU - Zhao, Jie
AU - Zhu, Changhua
AU - Gan, Lijun
AU - Yu, Jinping
AU - Witte, Claus Peter
AU - Chen, Mingjia
N1 - Funding Information: The authors thank Jialei Wang for her help with mass spectrometry analyses, Christel Schmiechen for her assistance with seedling phenotype analyses, and Markus Niehaus for his help with statistical analyses. This work was financially supported by the Fundamental Research Funds for the Central Universities [grant number YDZX2023013]; the National Natural Science Foundation of China [grant number 31900907]; the Natural Science Foundation of Jiangsu Province, China [grant number BK20190528]; the International Centre for Genetic Engineering and Biotechnology [grant number CRP/CHN20-04_EC]; the Deutsche Forschungsgemeinschaft (DFG) [grant numbers CH2292/1-1 to M.C. and C.-P.W, WI3411/8-1, and INST 187/741-1 FUGG to C.-P.W.]; the Special Fund on Technology Innovation of Carbon Dioxide Peaking and Carbon Neutrality of Jiangsu Province [grant number BE2022306 to J.Y.]; and the start-up fund for advanced talents from Nanjing Agricultural University to M.C.
PY - 2023/10
Y1 - 2023/10
N2 - The biological function of RNA can be modulated by base modifications. Here, we unveiled the occurrence of N4-acetylation of cytidine in plant RNA, including mRNA, by employing LC-MS/MS and acRIP-seq. We identified 325 acetylated transcripts from the leaves of 4-week-old Arabidopsis (Arabidopsis thaliana) plants and determined that 2 partially redundant N-ACETYLTRANSFERASEs FOR CYTIDINE IN RNA (ACYR1 and ACYR2), which are homologous to mammalian NAT10, are required for acetylating RNA in vivo. A double-null mutant was embryo lethal, while eliminating 3 of the 4 ACYR alleles led to defects in leaf development. These phenotypes could be traced back to the reduced acetylation and concomitant destabilization of the transcript of TOUGH, which is required for miRNA processing. These findings indicate that N4-acetylation of cytidine is a modulator of RNA function with a critical role in plant development and likely many other processes.
AB - The biological function of RNA can be modulated by base modifications. Here, we unveiled the occurrence of N4-acetylation of cytidine in plant RNA, including mRNA, by employing LC-MS/MS and acRIP-seq. We identified 325 acetylated transcripts from the leaves of 4-week-old Arabidopsis (Arabidopsis thaliana) plants and determined that 2 partially redundant N-ACETYLTRANSFERASEs FOR CYTIDINE IN RNA (ACYR1 and ACYR2), which are homologous to mammalian NAT10, are required for acetylating RNA in vivo. A double-null mutant was embryo lethal, while eliminating 3 of the 4 ACYR alleles led to defects in leaf development. These phenotypes could be traced back to the reduced acetylation and concomitant destabilization of the transcript of TOUGH, which is required for miRNA processing. These findings indicate that N4-acetylation of cytidine is a modulator of RNA function with a critical role in plant development and likely many other processes.
UR - http://www.scopus.com/inward/record.url?scp=85172739894&partnerID=8YFLogxK
U2 - 10.1093/plcell/koad189
DO - 10.1093/plcell/koad189
M3 - Article
C2 - 37367221
AN - SCOPUS:85172739894
VL - 35
SP - 3739
EP - 3756
JO - PLANT CELL
JF - PLANT CELL
SN - 1040-4651
IS - 10
ER -