Details
| Original language | English |
|---|---|
| Pages (from-to) | 661-666 |
| Number of pages | 6 |
| Journal | Journal of Nutritional Biochemistry |
| Volume | 26 |
| Issue number | 6 |
| Publication status | Published - 1 Jun 2015 |
Abstract
In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.
Keywords
- Glucoerucin, Glucosinolates, Heme oxygenase 1, MAPK, Myrosinase, Nrf2
ASJC Scopus subject areas
- Medicine(all)
- Endocrinology, Diabetes and Metabolism
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Nursing(all)
- Nutrition and Dietetics
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
Sustainable Development Goals
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In: Journal of Nutritional Biochemistry, Vol. 26, No. 6, 01.06.2015, p. 661-666.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Myrosinase-treated glucoerucin is a potent inducer of the Nrf2 target gene heme oxygenase 1 - studies in cultured HT-29 cells and mice
AU - Wagner, A.E.
AU - Sturm, C.
AU - Piegholdt, S.
AU - Wolf, I.M.A.
AU - Esatbeyoglu, T.
AU - De Nicola, G.R.
AU - Iori, R.
AU - Rimbach, G.
N1 - Funding information: This work was supported by a grant from the “Deutsche Forschungsgemeinschaft (DFG)” to A.E.W. (WA 3370/1-1) and by the DFG cluster of excellence “Inflammation at Interfaces”.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.
AB - In this study, the effect of myrosinase-treated glucoerucin (GER+MYR), which releases the isothiocyanate (ITC) erucin, on heme oxygenase 1 (HO-1) gene expression and Nrf2 signaling was investigated in vitro in cultured cells and in vivo in mice. Treatment of HT-29 cells with GER+MYR resulted in a significant increase in the mRNA and protein levels of nuclear Nrf2 and HO-1. GER+MYR was more potent at enhancing the nuclear Nrf2 levels than were the following myrosinase-treated glucosinolates: sinigrin, glucoraphanin and gluconasturtiin, which are the precursors of allyl-ITC, R-sulforaphane and 2-phenylethyl ITC, respectively. GER+MYR also significantly induced HO-1 gene expression in the mouse intestinal mucosae and liver but not in the brain. Mechanistic studies suggest that GER+MYR induces Nrf2 via ERK1/2-, p38- and JNK-dependent signal transduction pathways. The GER+MYR-mediated increase in HO-1 expression is primarily attributable to p38 signaling.
KW - Glucoerucin
KW - Glucosinolates
KW - Heme oxygenase 1
KW - MAPK
KW - Myrosinase
KW - Nrf2
UR - http://www.scopus.com/inward/record.url?scp=84928926277&partnerID=8YFLogxK
U2 - 10.1016/j.jnutbio.2015.01.004
DO - 10.1016/j.jnutbio.2015.01.004
M3 - Article
VL - 26
SP - 661
EP - 666
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
IS - 6
ER -